RECENT PUBLICATIONS

2024,2023, 2022, 2021

2020, 2019, 2018, 2017, 2016, 2015, 2014, 2013, 2012, 2011

2010, 2009, 2008, 2007, 2006, 2005, 2004, 2003, 2002, 2001

2000, 1999, 1998, 1997, 1996, 1995, 1994, 1993, 1992, 1991
1990, 1989, 1988, 1987, 1986, 1985, 1984, 1982, 1981, 1980, 1979, 1977, 1975

2024

S. Chaikaew, Y. Watanabe, D. J. Zheng, F. Motojima, T. Yamaguchi, and Y. Asano
Structure-Based Site-Directed Mutagenesis of Hydroxynitrile Lyase from Cyanogenic Millipede, Oxidus gracilis for hydrocyanation and Henry reactions, ChemBioChem, in press.

K. Mutoh, G. Iwasaki, Y. Asano, and K. Okuhara
Development of EC number prediction model using machine learning to predict the best enzyme for a chemical reaction (in Japanese), Seibutsukougakukaishi, 102, 4 (2024).

T. Yamaguchi, Y. Asano
Nitrile-synthesizing enzymes and biocatalytic synthesis of volatile nitrile compounds: A review, J. Biotechnol., 384, 20-28 (2024).

2023

T. Yamaguchi, T. Nomura, and Y. Asano
Identification and characterization of cytochrome P450 CYP77A59 of loquat (Rhaphiolepis bibas) responsible for biosynthesis of phenylacetonitrile, a floral nitrile compound, Planta, 257(6), 114 (2023). doi: 10.1007/s00425-023-04151-x.

2022

S. Shinoda, A. Itakura, H. Sasano, R. Miyake, H. Kawabata, and Y. Asano
Rational design of the soluble variant of L-pipecolic acid hydroxylase using the a-helix rule and hydropathy contradiction rule, ACS Omega, 7(33), 29508-29516 (2022). published on line on Aug 11, 2022. [Open Access] doi.org/10.1021/acsomega.2c04247

N. Kawahara, K. Palasin, and Y. Asano
Novel enzymatic method for imine synthesis via the oxidation of primary amines using D-amino acid oxidase from porcine kidney, Catalysts, 12(5), 511 (2022). [Open Access] https://doi.org/10.3390/catal12050511.

D. Matsui, N. Muraki, K. Chen, T. Mori, A.A. Ingram, K. Oike, H. Groger, S. Aono, and Y. Asano
Crystal structural analysis of aldoxime dehydratase from Bacillus sp. OxB-1: Importance of surface residues in optimization for crystallization, J. Inorg. Biochem., 230, 111770 (2022). [Open Access] https://doi.org/10.1016/j.jinorgbio.2022.111770

D.-J. Zheng, M. Nakabayashi, and Y. Asano
Structural characterization of Linum usitatissimum hydroxynitrile lyase: a new cyanohydrin decomposition mechanism involving a cyano-zinc complex. J. Biol. Chem., 298(3), 101650 (2022).[Open Access] doi: 10.1016/j.jbc.2022.101650.

Y. Hayashi, M. Nakamura, S. Nakano, S. Ito, Y Asano, and D. Sugimori
Thermostability enhancement of l-glutamate oxidase from Streptomyces sp. NT1 by full consensus protein design. J. Biosci. Bioeng., 133(4), 309-315 (2022). published on line on Jan 19, 2022 https://doi.org/10.1016/j.jbiosc.2021.12.008

C. Peel, S. Shinoda, and Y. Asano
Examination of the substrate scope of prenylated flavin mononucleotide-dependent ferulic acid decarboxylase from Aspergillus niger. Bull. Toyama Pref. Univ., 32, 59-64 (2022).

T. Yamaguchi, and Y. Asano
Construction of the UDP-glucose biosynthetic enzyme gene co-expression plasmid for prunasin production in Escherichia coli, In Plant Specialized Metabolism - Methods in Molecular Biology, Ed. A. G. Fett-Neto, Springer Nature, pp19-28 (2022).

Y. Asano
Discovery and structure of hydroxynitrile and related enzymes in the Aldoxime nitrile pathway of millipedes (in Japanese), Journal of Japanese Biochemical Society, 94(5), 681-689 (2022). doi:10.14952/SEIKAGAKU.2022.940681

K. Suzuki, K. Sakakibara, M. Nakamura, S. Shinoda, and Y. Asano
Machine Learning for Protein Solubility Prediction, 2022 International Conference on Machine Learning and Cybernetics (ICMLC), Japan, 237-241 (2022). doi: 10.1109/ICMLC56445.2022.9941322.

2021

D.-J. Zheng, and Y. Asano
A cyanide-free biocatalytic process for synthesis of complementary enantiomers of 4-chloro-3-hydroxybutanenitrile from allyl chloride, ChemCatChem, 13, 4237-4242 (2021). [Open Access] doi/10.1002/cctc.202100835

D. Matsui, Y. Hirata, A. Iwakawa, Y. Toyotake, M. Wakayama, and Y. Asano
Combination of enzymatic oxidation of amino acid and native chemical ligation with hydroxylamine for amide formation toward a one-pot process, Chem. Lett., 50 (9), 1632-1634 (2021). doi.org/10.1246/cl.210286

K. Kozuka, S. Nakano, Y. Asano, and S. Ito
Partial consensus design and enhancement of protein function by secondary structure-guided consensus mutations, Biochemistry, 60(29), 2309-2319 (2021). DOI: 10.1021/acs.biochem.1c00309

K. Oike, J. Spros, D. Matsui, Y. Asano, and H. Groger
Protein engineering of the aldoxime dehydratase from Bacillus sp. OxB-1 based on a rational sequence alignment approach, Sci. Rep., 11(1), 14316 (2021). [Open Access] doi.org/10.1038/s41598-021-92749-0

H. Yavuzer, Y. Asano, and H. Groger
Rationalizing the unprecedented stereochemistry of an enzymatic nitrile synthesis through a combined computational and experimental approach, Angew. Chem. Int. Ed., 60(35), 19162-19168 (2021). published online on 22 April 2021. [Open Access] https://doi.org/10.1002/ange.202017234

D. Matsui, Y. Okayama, Y. Yamamoto, Y. Miyauchi, Z.-y. Zhai, and Y. Asano
Identification of L-histidine oxidase activity in Achromobacter sp. TPU 5009 for L-histidine determination, J. Biosci. Bioeng.,131(5), 469-474 (2021). DOI: 10.1016/j.jbiosc.2020.12.006

T. Yamaguchi, Y. Matsui, N. Kitaoka, Y. Kuwahara, Y. Asano, H. Matsuura, Y. Sunohara, and H. Matsumoto
A promiscuous fatty acid w-hydroxylase CYP94A90 is likely involved in biosynthesis of a floral nitro compound in loquat (Eriobotrya japonica), New Phytologist, 231(3), 1157-1170 (2021). doi: 10.1111/nph.17441.

A. Hinzmann, T. Betke, Y. Asano, and H. Groeger
Synthetic processes toward nitriles without the use of cyanide: A biocatalytic concept based on dehydration of aldoximes in water, Chem. Eur. J., 27, 5313-5321 (2021). published online on 28 Oct. 2020. doi.org/10.1002/chem.202001647

T. Motoyama, N. Hiramatsu, Y. Asano, S. Nakano, and S. Ito
Protein sequence selection method that enables full consensus design of artificial threonine dehydrogenases with unique enzymatic properties, Biochemistry, 59(40), 3823-3833 (2021). published online on September 18, 2020. https://doi.org/10.1021/acs.biochem.0c00570

F. Motojima, A. Izumi, A. Nuylert, Z.-Y. Zhai, M. Dadashipour, S. Shichida, T. Yamaguchi, S. Nakano, and Y. Asano
R-hydroxynitrile lyase from the cyanogenic millipede, Chamberlinius hualienensis -A new entry to the carrier protein family Lipocalines -, FEBS J., 288(5), 1679-1695 (2021). published online on July 17, 2020. [Early View], [Open Access] https://doi.org/10.1111/febs.15490

Y. Asano
Frontier of flavoenzymes and applied enzymology, In Encyclopedia of Vitamin and Biofactors (Asakura Press) (in Japanese), 146-149 (2021).

Ke Chen, Zhongqiang Wang, Kan Ding, Yongzheng Chen, and Yasuhisa Asano
Recent progress on discovery and research of aldoxime dehydratases, Green Synthesis and Catalysis, 2(2), 179-186 (2021). [Open Access] 10.1016/j.gresc.2021.04.001

2020

A. Nuylert, M. Nakabayashi, T. Yamaguchi, and Y. Asano
Discovery and structural analysis to improve the enantioselectivity of hydroxynitrile lyase from Parafontaria laminata millipedes for (R)-2-chloromandelonitrile synthesis, ACS Omega, 5(43), 27896-27908 (2020). published online on October 15, 2020.[Open Access] [Cover picture], https://doi.org/10.1021/acsomega.0c03070

D.-j. Zheng, and Y. Asano
Biocatalytic asymmetric ring-opening of dihydroisoxazoles: a cyanide-free route to complementary enantiomers of ﾎｲ-hydroxy nitriles from olefins, Green Chem., 22, 4930-4936 (2020). [Open Access], [2020 Green Chemistry Hot Article] DOI: 10.1039/D0GC01445A

D. Trisrivirat, N. Lawan, P. Chenprakhon, D. Matsui, Y. Asano, and P. Chaiyen
Mechanistic insights into the dual activities of the single active site of L-lysine oxidase/monooxygenase from Pseudomonas sp. AIU 813, J. Biol. Chem., 295(32), 11246-11261 (2020). published on line on 11 Jun, 2020, doi:10.1074/jbc.RA120.014055

J. Gimenez-Dejoz, K. Tsuchiya, A. Tateishi, Y. Motoda,T. Kigawa, Y. Asano, and K. Numata
Computational study on the polymerization reaction of D-Aminopeptidase for the synthesis of D-peptides, RSC Chemical Biology, 10, 17582-17592 (2020). [Open Access] 10.1039/D0RA01138J

H. Groeger, and Y. Asano
Cyanide-free enantioselective catalytic strategies for the synthesis of chiral nitriles, J. Org. Chem., 85(10), 6243-6251 (2020). doi: 10.1021/acs.joc.9b02773.

Ji Eun Choi, S. Shinoda, Y. Asano, and H. Groeger
Aldoxime dehydratase mutants as improved biocatalysts for a sustainable synthesis of biorenewables-based 2-furonitrile, Catalysts 10(4), 362 (2020). [Open Access] https://doi.org/10.3390/catal10040362

A. Nuylert, F. Motojima, C. Khanongnuch, T. Hongpattarakere, and Y. Asano
Stabilization of hydroxynitrile lyases from two variants of passion fruits, Passiflora edulis Sims and Passiflora edulis forma flavicarpa by C-terminal truncation, ChemBioChem, 21(1-2), 181-189 (2020). doi: 10.1002/cbic.201900468.

K. Fukaya, Y. Kono, M. Hibi, Y. Asano, and D. Urabe, A New Entry to the Synthesis of (±)-b-Lysine, Heterocycles, 101, 701-706 (2020). 10.3987/COM-19-S(F)38

Yuki OZAKI, Tatsuki YAMAZAKI, Yuki TSUCHIYA, Ryo TSUGITA, Masaki NAKAMURA, Kazutoshi SAKAKIBARA, Suguru SHINODA, and Yasuhisa ASANO
Formal concept analysis of properties of proteins based on amino-acid sequences and structure information, TECHNICAL REPORT OF IEICE (in Japanese). 119, 470 , 5-8 (2020).

K. Yasukawa, N. Kawahara, F. Motojima, S. Nakano, and Y. Asano
Porcine kidney D-amino acid oxidase-derived R-amine oxidases with new substrate specificities, In The Enzymes, Flavin-Dependent Enzymes, mechanisms, structures and applications, 47, 117-136 (2020). 10.1016/bs.enz.2020.06.007

K. Yasukawa, and Y. Asano
Amine oxidase-catalysed deracemisation of (R,S)-4-Cl-benzhydrylamine into the (R)-enantiomer in the presence of a chemical reductant, In Applied Biocatalysis: The Chemist's Enzyme Toolkit, published online (2020). 10.1002/9781119487043

2019

J. E. Choi, S. Shinoda, R. Inoue, D. Zheng, H. Groger, and Y. Asano
Cyanide-free synthesis of an aromatic nitrile from a biorenewable-based aldoxime: Development and application of a recombinant aldoxime dehydratase as a biocatalyst. Biocat. Biotransf. 37, 414-420 (2019).

C. Plass, A. Hinzmann, M. Terhorst, W. Brauer, K. Oike, H. Yavuzer, Y. Asano, A. Vorholt, T. Betke, and H. Groeger
Approaching bulk chemical nitriles from alkenes: A hydrogen cyanide-free approach through a combination of hydroformylation and biocatalysis, ACS Catal., 9 (6) 5198-5203 (2019).

M. Tatsumi, W. Hoshino, Y. Kodama, T. Ueatrongchit, K. Takahashi, U. Tagami, H. Yamaguchi, H. Miyano, Y. Asano, and T. Mizukosh
Development of a rapid and simple glycine analysis method using a stable glycine oxidase mutant, Anal. Biochem., 587, 113447 (2019). doi:10.1016/j.ab.2019.113447

K. Isobe, D. Matsui, and Y. Asano
Comparative review of the recent enzymatic methods used for selective assay of L-lysine, Anal. Biochem., 584, 113335 (2019). DOI: 10.1016/j.ab.2019.06.006

Y. Takakura, and Y. Asano
Purification, characterization, and gene cloning of a novel aminoacylase from Burkholderia sp. strain LP5_18B that efficiently catalyzes the synthesis of N-lauroyl-L-amino acids, Biosci. Biotechnol. Biochem., 83 (8),(2019). Published online: 14 Jun 2019

D. Matsui, and Y. Asano
Creation of thermostable L-tryptophan dehydrogenase by protein engineering and its application for L-tryptophan quantification, Anal. Biochem., 579, 57-63 (2019).

Y. Kuwahara, Y. Ichiki, M. Morita, T. Tanabe, R. Nakata, N. Mori, and Y. Asano
Defensive secretion of Oxidus gratilis (C.L. Koch) (Paradoxosomatidae: Polydesma): Juvenile-adult and juvenile-juvenile polymorphism, sexal maturation and sexual dimorphism, Jpn. J. Environ. Entomol. Zoo., 30 (2), 39-49 (2019).

Z.-Y. Zhai, A. Nuylert, K. Isobe, and Y. Asano
Effects of codon optimization and glycosylation on the high-level production of hydroxynitrile lyase from Chamberlinius hualienensis in Pichia pastoris, J. Ind. Microbiol. Biotechnol., 46 (7), 887-898 (2019). On line on 16 March 2019

Y. Asano
Screening and development of enzymes for determination and transformation of amino acids, Biosci. Biotech. Biochem., 83 (8), 1402-1416 (2019). Published online: 08 Jan 2019. doi.org/10.1080/09168451.2018.1559027

Y. Asano, and K. Yasukawa
Identification and development of amino acid oxidases, Current Opinion in Chemical Biology, 49, 76-83 (2019). Available online 15 November 2018. doi.org/10.1016/j.cbpa.2018.10.020

Y. Asano
Development of microbial, plant and animal enzymes and their basis for application (in Japanese), Seibutsu-Kogaku Kaishi, 97, 3-12 (2019).

S. Nakano, M. Niwa, S. Ito, Y. Asano
Analysis of evolution of L-arginine oxidase with high affinity toward L-arginine (in Japanese), Enzyme Egnineering News, 82, 18-21 (2019).

Y. Asano
Recent advance in the enzymatic determination of amino acid and related compounds, Editorial for the special issue on enzyme assay of amino acids, Anal. Biochem. 587, 113464 (2019). doi.org/10.1016/j.ab.2019.113464

T. Motoyama, S. Nakano, H. Tokiwa, Y. Asano, and S. Ito
In silico screening and product release mechanism of monomeric L-threonine 3-dehydrogenase (in Japanese), AgriBio, 2 (11), 47-48 (2019).

2018

A. Nuylert, Y. Kuwahara, T. Hongpattarakere, and Y. Asano
Identification of saturated and unsaturated 1-methoxyalkanes from the Thai millipede Orthomorpha communis as potential "Raincoat Compounds", Sci. Rep., 8, 11730 (2018). doi: 10.1038/s41598-018-30156-8. [Open Access]

H. Yamaguchi, K. Takahashi, M. Tatsumi, U. Tagami, M. Sugiki, T. Kashiwagi, M. Kameya, S. Okazaki, T. Mizukoshi and Y. Asano
Protein engineering for improving the thermostability of tryptophan oxidase and insights from structural analysis, J. Biochem., 164 (5), 359-367 (2018). doi: 10.1093/jb/mvy065.

Y. Kuwahara, and Y. Asano
Generation of (2-nitroethyl)benzene and related benzenoids from L-Phenylalanine; Flower scents of the Japanese loquat Eriobotrya japonica [Rosales: Rosaceae], Biosci. Biotech. Biochem., 82 (11), 1855-1866 (2018). doi.org/10.1080/09168451.2018.1498319

T. Yamaguchi, and Y. Asano
Prunasin production using engineered Escherichia coli expressing UGT85A47 from Japanese apricot and UDP-glucose biosynthetic enzyme genes, Biosci. Biotech. Biochem., 82 (10), 2021-2029 (2018). doi.org/10.1080/09168451.2018.1497942.

K. Isobe, A. Kitagawa, K. Kanamori, N. Kashiwagi, D. Matsui, T. Yamaguchi, K. Fuhshuku, H. Semba, and Y. Asano
Characterization of a novel hydroxynitrile lyase from Nandina domestica Thunb., Biosci. Biotech. Biochem., 82 (10),1760-1769 (2018). doi.org/10.1080/09168451.2018.1490171

K. Yasukawa, F. Motojima, A. Ono, and Y. Asano
Expansion of the substrate specificity of porcine kidney D-amino acid oxidase for S-stereoselective oxidation of 4-Cl-benzhydrylamine, ChemCatChem, 10 (16), 3500-3505 (2018). doi.org/10.1002/cctc.201800614. [Open Access]

Y. Kuwahara, Y. Ichiki, M. Morita, T. Tanabe, and Y. Asano
Biosynthesis of (2-nitroethyl)benzene and (Z)- and (E)-(2-nitroethenyl)benzenes from (Z)- and (E)-phenylacetaldoximes and phenylacetonitrile; defense allomone of Eutrichodesmus elegans and Eutrichodesmus armatus (Polydesmida: Haplodesmidae), J. Pest. Sci., 43 (4), 240-247 (2018). DOI: 10.1584/jpestics.D17-098

S. Nakano, T. Motoyama, Y. Miyashita, Y. Ishizuka, T. Matsuo, H. Tokiwa, S. Shinoda, Y. Asano, and S. Ito
Benchmark analysis of native and artificial NAD⁺-dependent enzymes generated by a sequence based design method with or without phylogenetic data, Biochemistry, 57 (26), 3722-3732 (2018). doi: 10.1021/acs.biochem.8b00339

P. Sakpetch, H. Komeda, Y. Asano, Y. Kuwahara, and Aran H-kittikun
Isolation of indigenous antagonistic microorganism to inhibit Rigidoporus microporus and other plant pathogens and analysis of the bioactive compounds. Biological Control, 124, 53-60 (2018). doi.org/10.1016/j.biocontrol.2018.01.007

T. Yamaguchi, A. Nuylert, A. Ina, T. Tanabe, and Y. Asano
Hydroxynitrile lyases from cyanogenic millipedes: molecular cloning, heterologous expression, and whole-cell biocatalysis for the production of (R)-mandelonitrile, Sci. Rep., 8, 3051 (2018). DOI:10.1038/s41598-018-20190-x. [Open Access]

Dohyun Im, D. Matsui, T. Arakawa, K. Isobe, Y. Asano, and S. Fushinobu
Ligand complex structures of L-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813 and its conformational change, FEBS Open Bio, doi:10.1002/2211-5463.12387 [Cover picture], [Open Access]

T. Betke, J. Higuchi, P. Rommelmann, K. Oike, T. Nomura, Y. Kato, Y. Asano, and H. Groger
Biocatalytic synthesis of nitriles through dehydration of aldoximes: the substrate scope of aldoxime dehydratases, ChemBioChem, 19 (8), 768-779 (2018). Accepted manuscript online: 14 January 2018, DOI: 10.1002/cbic.201700571 [Cover Picture]

S. Srinuanpan, B. Cheirsilp, P. Prasertsan, Y. Kato, Y. Asano
Strategies to increase the potential use of oleaginous microalgae as biodiesel feedstocks: Nutrient starvations and cost-effective harvesting process, Renewable Energy, 122, 507-516 (2018). doi.org/10.1016/j.renene.2018.01.121

S. Srinuanpan, B. Cheirsilp, P. Prasertsan, Y. Kato, Y. Asano
Photoautotrophic cultivation of oleaginous microalgae and co-pelletization with filamentous fungi for cost-effective harvesting process and improved lipid yield, Aquaculture International, 26, 1493-1509 (2018).

K. Isobe, S. Miki, R. Ueda, S. Shichida, D. Matsui, Y. Oku, and Y. Asano
Characterization of two carbonyl reductases from Ogataea polymorpha NBRC 0799, Appl. Microbiol. Biotechnol., 102 (3), 1307-1316 (2018). First Online: 13 December 2017, doi.org/10.1007/s00253-017-8668-8

Y. Ishida, V. B. Meyer-Rochow, and Y. Asano
Comparison of DNA sequence encoding hydroxynitrile lyase from the invasive millipede, Chamberlinius hualienensis, collected at Kagoshima, Shizuoka, and Hachijo, Tokyo, Bull. Toyama Pref. Univ., 28, 35-40 (2018).

Y. Asano, and R. Metzner
Exploiting natural diversity for industrial enzymatic applications, In Modern Biocatalysis -Advances toward Synthetic Biological Systems-, Royal Society of Chemistry, Catalysis Series, Ed. Gavin Williams and Mellanie Hall, Chapter 2, pp 28-52 (2018).

Y. Asano, and M. Araki
Enzyme engineering based on structural informatics (in Japanese), Seibutsu-Kogaku Kaishi, 96 (10), 568 (2018).

D. Matsui, S. Nakano, M. Dadashipour, and Y. Asano
Rational identification of aggregation hotspots based on secondary structure and amino acid hydrophobicity (in Japanese), Enzyme Engineering News, 80, 14-18 (2018).

D. Matsui, and Y. Asano
Soluble expression of the genes for enzymes by rational mutagenesis (in Japanese), Seibutsu-Kogaku Kaishi, 96 (10), 569-572 (2018).

2017

F. Motojima, A. Nuylert, and Y. Asano
The crystal structure and catalytic mechanism of hydroxynitrile lyase from passion fruit, Passiflora edulis, FEBS J., 285 (2), 313-324 (2018). Published online, 7 December 2017. DOI: 10.1111/febs.14339 [Open Access]

T. Motoyama, S. Nakano, Y. Yamamoto, H. Tokiwa, Y. Asano, and S Ito
Product release mechanism associated with structural changes in monomeric L-threonine 3-dehydrogenase, Biochemistry, 56 (43), 5758-5770 (2017) DOI: 10.1021/acs.biochem.7b00832, Published online, 9 October 2017.

T. Betke, P. Rommelmann, K. Oike, Y. Asano, and H. Groger
Cyanide-free and broadly applicable enantioselective synthetic platform for chiral nitriles through a biocatalytic approach, Angew. Chem. Int. Ed., 56 (40), 12361-12366 (2017). DOI: 10.1002/anie.201702952 [Back Cover Picture], [Very Important Paper] ^*

D. Matsui, K. Fuhshuku, S. Nagamori, M. Takata, and Y. Asano
Isolation and characterization of racemase from Ensifer sp. 23-3 that acts on a-aminolactams and a-amino acid amides J. Ind. Microbiol. Biotechnol., 44 (11), 1503-1510 (2017). doi:10.1007/s10295-017-1981-5

D. Matsui, S. Nakano, M. Dadashipour, and Y. Asano
Rational identification of aggregation hotspots based on secondary structure and amino acid hydrophobicity, Sci. Rep., 7, 9558 (2017). DOI:10.1038/s41598-017-09749-2 [Open Access]

K. Yamamoto, Y. Oku, A. Ina, A. Izumi, M. Doya, S. Ebata, and Y. Asano
Purification and characterization of a novel enone reductase from Sporidiobolus salmonicolor TPU 2001 reacting with large monocyclic enones, ChemCatChem, 9, 3697-3704 (2017). DOI: 10.1002/cctc.201700244

S. Yamasaki-Yashiki, H. Komeda, K. Hoshino, and Y. Asano
Characterization and gene cloning of L-xylulose reductase involved in L-arabinose catabolism from the pentose-fermenting fungus Rhizomucor pusillus, Biosci. Biotechnol. Biochem., 81 (8), 1612-1618 (2017). http://dx.doi.org/10.1080/09168451.2017.1320518

K. Yamamoto and Y. Asano
Genome sequence of a lumichrome producer, Microbacterium sp. strain TPU 3598, Genome announcement, 5 (16), e00204-17 (2017).

Y. Kuwahara, T. Tanabe, and Y. Asano
Changes in defense allomone compositions of Eutrichodesmus elegans and Eutrichodesmus armatus (Polydesmida: Haplodesmidae) during different stages of their life cycles. Appl. Entomol. Zool., 52(2), 305-412 (2017). DOI: 10.1007/s13355-017-0478-z

Y. Kuwahara, M. Morita, Y. Ichiki, T. Tanabe, and Y. Asano
1-Phenyl-2-pentanone and methyl salicylate: new defense allomone components and their content shift during ontogenetic development of the millipede Nedyopus tambanus mangaesinus (Polydesmida: Paradoxosomatidae). Appl. Entomol. Zool., 52(3), 447-455 (2017). doi: 10.1007/s13355-017-0496-x

Y. Kuwahara, T. Tanabe, and Y. Asano
Defensive allomone components of the millipede Cryptocorypha kumamotensis (Murakami, 1966) [Polydesmida: Pyrgodesmidae] and two related Cryptocorypha spp.. J. Environ. Entomol. Zool. 28(2), 63-69 (2017). https://doi.org/10.11257/jjeez.28.63

Y. Kuwahara, T. Tanabe, and Y. Asano
Defensive allomone components of an unidentified myrmecophilous millipede in the genus Ampelodesmus ("hagayasude" in Japanese) [Polydesmida: Pyrgodesmidae] Jpn. J. Environ. Entomol. Zool. 28(2), 55-62 (2017). https://doi.org/10.11257/jjeez.28.55

Y. Kuwahara, T. Yamaguchi, Y. Ichiki, T. Tanabe, Y. Asano
Hydrogen peroxide as a new defensive compound in "benzoyl cyanide" producing polydesmid millipedes, Sci. Nat., 104,19, (2017) doi:10.1007/s00114-017-1435-y

Y. Miki, S. Okazaki, and Y. Asano
Engineering an ATP-dependent D-Ala:D-Ala ligase for synthesizing amino acid amides from amino acids, J. Ind. Microbiol. Biotechnol., 44 (4), 667-675 (2017). DOI 10.1007/s10295-016-1833-8

W. Payoungkiattikun, S. Okazaki, A. Ina, A. H-Kittikun, and Y. Asano
Characterization of an a-amino-e-caprolactam racemase with broad substrate specificity from Citreicella sp. SE45, J. Ind. Microbiol. Biotechnol., 44 (4), 677-685 (2017). DOI: 10.1007/s10295-016-1825-8.

Y-.f. Miao, R. Metzner, and Y. Asano
Kemp elimination catalyzed by naturally occurring aldoxime dehydratases, ChemBioChem, 18 (5), 451-454 (2017). DOI: 10.1002/cbic.201600596

A. Nuylert, Y. Ishida, and Y. Asano
Effect of glycosylation on the biocatalytic properties of hydroxynitrile lyase from the passion fruit, Passiflora edulis - a comparison of natural and recombinant enzymes, ChemBioChem, 18 (3), 257-265 (2017). DOI: 10.1002/cbic.201600447.

M. Kameya and Y. Asano
Translation-dependent bioassay for amino acid quantification using auxotrophic microbes as biocatalysts of protein synthesis, Appl. Microbiol. Biotechnol, 101 (6), 2523-2531 (2017). doi:10.1007/s00253-016-8027-1

T. Yamaguchi, Y. Kuwahara, and Y. Asano
A novel cytochrome P450, CYP3201B1, is involved in (R)-mandelonitrile biosynthesis in a cyanogenic millipede, FEBS Open Bio, 7 (3), 335-347 (2017). DOI: 10.1002/2211-5463.12170 [Open Access]l

W. Sukpipat, H. Komeda, P. Prasertsan, and Y. Asano
Purification and characterization of xylitol dehydrogenase with L-arabitol dehydrogenase activity from the newly isolated pentose-fermenting yeast Meyerozyma caribbica 5XY2, J. Biosci. Bioeng., 123 (1), 20-27 (2017).

N. Kawahara, K. Yasukawa, and Y. Asano
New enzymatic methods for the synthesis of primary a-aminonitriles and unnatural a-amino acids by oxidative cyanation of primary amine with D-amino acid oxidase from porcine kidney, Green Chem.,19, 418-424 (2017). DOI: 10.1039/C6GC02003H [Hot Artcile in 2017 of Green Chemistry]

Y. Asano, and S. Okazaki
Enzymes in aldoxime-nitrile pathway: versatile tools in biocatalysis, In Future directions in bioctalysis, ver 2, Elsevier, New York, U.S.A., Ed. Tomoko Matsuda, Chapter 9, pp173-187 (2017).

Y. Asano and D. Matsui
Development of enzymes to determine amino acids by growth-dependent molecular selection (In Japanese), Fine Chemical, 46 (2), (2017).

2016

S. Nakano, K. Yasukawa, T. Tokiwa, T. Ishikawa, E. Ishitsubo, N. Matsuo, S. Ito, H. Tokiwa, and Y. Asano
Origin of stereoselectivity and substrate/ligand recognition in an FAD-dependent R-selective amine oxidase, J. Phys. Chem. B, 120 (41), 10736-10743 (2016). DOI: 10.1021/acs.jpcb.6b09328.

Y. Ishida, Y. Kuwahara, M. Dadashipour, A. Ina, T. Yamaguchi, M. Morita, Y. Ichiki, and Y. Asano
A sacrificial millipede altruistically protects its swarm using a drone blood enzyme, mandelonitrile oxidase, Sci. Rep., 6, 26998 (2016). doi:10.1038/srep26998 [Open Access]

T. Yamaguchi, and Y. Asano
Draft genome sequence of an aldoxime degrader, Rhodococcus sp. strain YH3-3, Genome announcements, 4(3), e00406-16 (2016). doi: 10.1128/genomeA.00406-16.

T. Yamaguchi, K. Noge, and Y. Asano
Cytochrome P450 CYP71AT96 catalyses the final step of herbivore-induced phenylacetonitrile biosynthesis in the giant knotweed, Fallopia sachalinensis, Plant Mol. Biol., 91 (3), 1-11 (2016). DOI: 10.1007/s11103-016-0459-6 [Cover picture] [Open Access]

D. Matsui, A. Terai, and Y. Asano
L-Arginine oxidase from Pseudomonas sp. TPU 7192: Characterization, gene cloning, heterologous expression, and application to L-arginine determination, Enzyme Microb. Technol., 82, 151-157 (2016). DOI: 10.1016/j.enzmictec.2015.10.002

Y. Asano and N. Kawahara
A new S-hydroxynitrile lyase from Baliospermum montanum-Its structure, molecular dynamics simulation, and improvement by protein engineering, Industrial Biotechnology, 12 (2), 91-97 (2016). [Cover picture]

Y. Asano, S. Okazaki, and K. Yasukawa
Recent Developments in Amino peptidases, oxidases and racemases, In "Biocatalysis-Green Technology", Ed. Ramesh Patel, John Wiley & Sons, Inc., Hoboken, New Jersey, 489-502 (2016).

Y. Asano
Enchanted by Enzyme Discovery -Developing biological power-(In Japanese), Modern Chemistry (Gendai Kagaku), 539 (2), 48-53 (2016).

2015

K. Yamamoto and Y. Asano
Efficient production of lumichrome by Microbacterium sp. Strain TPU 3598, Appl. Environ. Microbiol., 81 (21), 7360-7367 (2015). doi: 10.1128/AEM.02166-15

S. Nakano, S. Okazaki, E. Ishitsubo, N. Kawahara, H. Komeda, H. Tokiwa, and Y. Asano
Structural and computational analysis of peptide recognition mechanism of class-C type penicillin binding protein, alkaline D-peptidase from Bacillus cereus DF4-B, Sci. Rep., 5, 13836 (2015). doi:10.1038/srep13836 [Open Access]

M. Dadashipour, Y. Ishida, K. Yamamoto, and Y. Asano
Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede Chamberlinius hualienensis, Proc. Natl. Acad. Sci. USA, 112 (34), 10605-10610 (2015). ^*[Cover picture] doi: 10.1073/pnas.1508311112 [Open Access]

N. Kawahara and Y. Asano
Mutagenesis of an Asn156 residue in a surface region of S-selective hydroxynitrile lyase from Baliospermum montanum enhances catalytic efficiency and enantioselectivity, ChemBioChem, 16 (13), 1891-1895 (2015). Published online: 27 JUL 2015 DOI: 10.1002/cbic.201500225, ^*[Inside Cover picture]

W. Payoungkiattikun, S. Okazaki, S. Nakano, A. Ina, A. H-Kittikun, and Y. Asano
In silico identification for a-amino-e-caprolactam racemase by using Information on the structure and function relationship, Appl. Biochem. Biotechnol., 176 (5), 1303-1314 (2015). DOI 10.1007/s12010-015-1647-6

K. Fuhshuku, S. Watanabe, T. Nishii, A. Ishii, and Y. Asano
A novel S-enantioselective amidase acting on 3,3,3-trifluoro-2-hydroxy-2-methylpropanamide from Arthrobacter sp. S-2, Biosci. Biotechnol. Biochem., 79 (10), 1587-1596 (2015). DOI:10.1080/09168451.2015.1038216

D. Matsui, and Y. Asano
Heterologous production of L-lysine e-oxidase by directed evolution using a fusion reporter method, Biosci. Biotechnol. Biochem., 79 (9), 11473-1480 (2015). DOI:10.1080/09168451.2015.1023251

S. Nakano, and Y. Asano
Protein evolution analysis of S-hydroxynitrile lyase by complete sequence design utilizing the INTMSAlign software, Sci. Rep., 5, 8193 (2015). doi:10.1038/srep08193. [Open Access]

T. Yamaguchi, and Y. Asano
Complete genome of an aldoxime degrader, Bacillus sp. OxB-1, Genome announcements, 3 (1) e00025-15 (2015). doi:10.1128/genomeA.00025-15.

D. Matsui, S. Okazaki, M. Matsda, and Y. Asano
Enhancement of stability of L-tryptophan dehydrogenase from Nostoc punctiforme ATCC29133 and its application to L-tryptophan assay, J. Biotechnol., 196-197, 27-32 (2015). doi:10.1016/j.jbiotec.2015.01.010

A. Sugawara, D. Matsui, M. Yamada, Y. Asano, and K. Isobe
Characterization of two amine oxidases from Aspergillus carbonarius AIU 205, J. Biosci. Bioeng., 119 (6), 629-635 (2015). doi:10.1016/j.jbiosc.2014.10.023

A. Sugawara, D. Matsui, M. Yamada, Y. Asano, and K. Isobe
New enzymatic methods for selective assay of L-lysine using an L-lysine specific decarboxylase/oxidase from Burkholderia sp. AIU 395, J. Biosci. Bioeng., 119 (3), 369-374 (2015). doi:10.1016/j.jbiosc.2014.08.013

H. Komeda, S. Yamasaki-Yashiki, K. Hoshino, and Y. Asano
Identification and characterization of D-xylose reductase involved in pentose catabolism of the zygomycetous fungus Rhizomucor pusillus, J. Biosci. Bioeng., 119 (1), 57-64 (2015). DOI: 10.1016/j.jbiosc.2014.06.012

Y. Asano
Hydrolysis of Nitriles to Amides, In Science of Synthesis: Biocatalysis in Organic Synthesis, K. Faber, W.-D. Fessner, N. Turner, Eds, Georg Thieme Verlag KG., Vol. 1, pp 255-276 (2015).

2014

A. Sugawara, D. Matsui, M. Yamada, Y. Asano, and K. Isobe
Characterization of a pyridoxal-5'-phosphate-dependent l-lysine decarboxylase/oxidase from Burkholderia sp. AIU 395 J. Biosci. Bioeng., 118 (5), 496-501 (2014). doi:10.1016/j.jbiosc.2014.04.013

S. Nakano, M. Dadashipour, and Y. Asano
Structural and functional analysis of hydroxynitrile lyase from Baliospermum montanum with crystal structure, molecular dynamics and enzyme kinetics, BBA Proteins and Proteomics, 1844 (12), 2059-2067 (2014). doi:10.1016/j.bbapap.2014.09.004

H. Komeda, S. Yamasaki-Yashiki, K. Hoshino, and Y. Asano
Identification and characterization of D-xylulokinase from the D-xylose-fermenting fungus, Mucor circinelloides, FEMS Microbiol. Lett., 360 (1), 51-61 (2014). DOI: 10.1111/1574-6968.12589

R. Metzner, S. Okazaki, Y. Asano, and H. Groger
Cyanide-free enantioselective eynthesis of nitriles: synthetic proof of a biocatalytic concept and mechanistic insights, ChemCatChem, 6 (11), 3105-3109 (2014). DOI: 10.1002/cctc.201402612

Y. Miki, and Y. Asano
Construction of the biosynthetic pathway for the cyanide-free production of phenylacetonitrile in Escherichia coli utilizing plant cytochrome P450 79A2 and bacterial aldoxime dehydratase, Appl. Environ. Microbiol., 80 (21), 6828-6836 (2014) doi: 10.1128/AEM.01623-14 [Spotlight] (Articles of Significant Interest Selected from This Issue by the Editors).

T. Yamaguchi, K. Yamamoto, and Y. Asano
Identification and characterization of CYP79D16 and CYP71AN24 catalyzing the first and second steps in L-phenylalanine-derived cyanogenic glycoside biosynthesis in the Japanese apricot, Prunus mume Sieb. et Zucc, Plant Molec. Biol., 86 (1), 215-223 (2014). DOI:10.1007/s11103-014-0225-6

S. Yamasaki-Yashiki, H. Komeda, K. Hoshino, and Y. Asano
Molecular analysis of NAD⁺-dependent xylitol dehydrogenase from the zygomycetous fungus Rhizomucor pusillus and reversal of the coenzyme preference, Biosci. Biotechnol. Biochem., 78 (11),1943-1953 (2014). doi:10.1016/j.jbiosc.2014.06.012

Y. Kuwahara, Y. Ichiki, M. Morita, and Y. Asano
(2-Nitroethyl)benzene: a major flower scent from the Japanese Loquat Eriobotrya japonica [Rosales: Rosaceae], Biosci. Biotechnol. Biochem., 78 (8),1320-1323 (2014). DOI:10.1080/09168451.2014.921558

S. Nakano, S. Okazaki, H. Tokiwa, and Y. Asano
Binding of NAD⁺ and L-threonine induces stepwise structural and flexibility changes in Cupriavidus necator L-threonine dehydrogenase, J. Biol. Chem., 289 (15), 10445-10454 (2014). DOI: 10.1074/jbc.M113.540773

K. Yasukawa, S. Nakano, and Y. Asano
Tailoring D-amino acid oxidase from the pig kidney to R-stereoselective amine oxidase and its use in deracemization of a-methylbenzylamine, Angew. Chem. Int. Ed. Engl., 53 (17), 4428-4431 (2014). DOI: 0.1002/ange.201308812

A. Sugawara , D. Matsui, H. Komeda, Y. Asano, and K. Isobe
Characterization and application of aminoamide-oxidizing enzyme from Aspergillus carbonarius AIU 205, J. Biosci. Bioeng. 117 (3), 263-268 (2014). doi:10.1016/j.jbiosc.2013.08.019

D. Matsui, Do-Hyun Im, A. Sugawara, Y. Fukuta, S. Fushinobu, K. Isobe, and Y. Asano
Mutational and crystallographic analysis of L-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813: Interconversion between oxidase and monooxygenase activities, FEBS Open Bio, 4, 220-228 (2014). DOI: 10.1016/j.fob.2014.02.002, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970082/

K. Fuhshuku, M. Takata, H. Iwatsubo, and Y. Asano
Preparation of D-a-aminolactams by L-enantioselective degradation of a-aminolactam mediated by Mesorhizobium sp. L88, Biocatal. Agric. Biotechnol., 3 (3), 42-47 (2014). doi:10.1016/j.bcab.2014.01.003

K. Fuhshuku, S. Watanabe, T. Nishii, A. Ishii, Y. Asano
Efficient preparation of both enantiomers of 3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid catalyzed by Shinella sp. R-6 and Arthrobacter sp. S-2, J. Molec. Catal. B: Enzymatic., 102, 115-119 (2014). doi:10.1016/j.molcatb.2014.01.024

M. Kameya and Y. Asano
Rapid enzymatic assays for L-citrulline and L-arginine based on the platform of pyrophosphate detection, Enzyme Microb. Technol., 57 (10), 36-41 (2014). doi:10.1016/j.enzmictec.2014.01.008

M. Kameya, M. Himi, and Y. Asano
Rapid and selective enzymatic assay for L-methionine based on a pyrophosphate detection system, Anal. Biochem., 447, 33-38 (2014). https://doi.org/10.1016/j.ab.2013.11.002

S. Kudo, Y. Kuwahara, Z. Korsos, Y. Ichiki, M. Morita, Y. Asano, Y. Nakamura, and T. Tanabe
Notes on egg brooding newly recorded in two millipede species (in Japanese), Jpn. J. Ent (N.S.), 17 (4), 135-138 (2014).

Y. Asano, K. Yasukawa, S. Nakano
Development of a new amine oxidase which had not been found in Nature (in Japanese), Chemistry (Kagaku), 69 (8), 45-49 (2014).

Y. Asano
Industrial application of new mirobial and plant enzymes responsible for degradation and biosynthesis of nitriles (in Japanese), Chemistry and Biology (Kagaku to Seibutsu), 52 (10), 651-658 (2014).

Y. Asano, K. Yasukawa
Development of Enzymes for D-amino Acid Production, Bioindustry (in Japanese), 31(3), 49-56 (2014).

2013

S. Okazaki, S. Nakano, D. Matsui, S. Akaji, K. Inagaki and Y. Asano
X-Ray crystallographic evidence for the presence of the cysteine tryptophylquinone cofactor in L-lysine e-oxidase from Marinomonas mediterranea, J. Biochem., 154 (3), 233-236 (2013).

M. Kameya, H. Onaka, and Y. Asano
Selective tryptophan determination using tryptophan oxidases involved in bis-indole antibiotic biosynthesis, Anal. Biochem., 438 (2), 124-132 (2013).

Y. Asano
8.4.1 Biocalatlysis in Engineering: Basic knowledge (in Japanese), In "Kagaku Binran" (Handbook of Chemistry) (Maruzen Co., Ltd.), pp 489-492.

Y. Asano
D-stereospecific aminopeptidase, In N. D. Rawlings and G. S. Salvesen (ed.), Handbook of Proteolytic Enzymes 3rd Ed. (Academic Press, London), Vol. 3, Chapter 767, pp 3463-3467 (2013).

Y. Asano
Alkaline D-peptidase, In N. D. Rawlings and G. S. Salvesen (ed.), Handbook of Proteolytic Enzymes 3rd Ed. (Academic Press, London), Vol. 3, Chapter 768, pp 3467-3469 (2013).

2012

K. Yasukawa and Y. Asano
Enzymatic Synthesis of Chiral Phenylalanine Derivatives by a Dynamic Kinetic Resolution of Corresponding Amide and Nitrile Substrates with a Multi-Enzyme System, Advanced Synthesis & Catalysis, 354 (17), 3327-3332 (2012).

H. Takeuchi, E. Ito, T. Tomioka, E. Tabuchi, K. Fuhshuku, and Y. Asano
Trans fatty acid intake and serum cholesterol levels in young Japanese women, Biosci. Biotech. Biochem., 76 (9), 1627-1632 (2012).

K. Isobe, A. Sugawara, H. Domon, Y. Fukuta, and Y. Asano
Purification and characterization of an L-amino acid oxidase from Pseudomonas sp. AIU 813, J. Biosci. Bioeng., 114 (3), 257-261 (2012).

S. Yamasaki-Yashiki, S. Tachibana, and Y. Asano
Determination of L-methionine using methionine-specific dehydrogenase for diagnosis of homocystinuria due to cystathionine b-synthase deficiency, Anal. Biochem., 428 (2), 143-149 (2012). [Cover picture]

M. Matsuda and Y. Asano
A simple assay of taurine concentrations in food and biological sample using taurine dioxygenase, Anal. Biochem., 427 (2), 121-123 (2012). [Cover picture]

K. Fuhshuku, and Y. Asano
Synthesis of optically active medium-sized a-aminolactams via ring-closing metathesis, Tetrahedron, 68 (33), 6651-6655 (2012).

Harald Groger, Yasuhisa Asano, Uwe T. Bornscheuer, and Jun Ogawa
Development of Biocatalytic Processes in Japan and Germany - From Research Synergies to Industrial Applications, Chemistry-An Asian Journal, 7, 1138-1153 (2012), DOI: 10.1002/asia.201200105.

Y. Asano and P. Kaul
Hydrolysis and reverse hydrolysis: Selective nitrile hydrolysis using nitrile hydratase and nitrilase, In Comprehensive Chirality, (Elsevier), In "Comprehensive Chirality", Ed. E. M. Carreira and H. Yamamoto (Elsevier), Volume 7, pp 122-142.

H. Groger and Y. Asano
1. Introduction - Principle and historial landmarks of enzyme catalysis in organic synthesis. In "Enzyme Catalysis in Organic Synthesis", Ed. K. Drauz, H. Groger and O. May (Springer Verlag), Volume 1, pp 3-42 (2012).

Y. Asano and K. Hoelsch
39. Isomerizations. In "Enzyme Catalysis in Organic Synthesis", Ed. K. Drauz, H. Groger and O. May (Springer Verlag), Volume 3, pp 1607-1684 (2012).

K. Fuhshuku, and Y. Asano
Organic synthesis catalyzed by plant enzyme hydroxynitrile lyase (in Japanese), Japanese Journal of Synthetic Organic Chemistry, 50, 102-112 (2012).

Y. Asano
Enzymes acting on D-amino acid containing peptides. In L. Pollegioni and S. Servi (ed.) "Unnatural amino acids", "Methods in Molecular Biology" series 794 (Humana Press), pp 397-406 (2012).

2011

Eftychia Pinakoulaki, Constantinos Koutsoupakis, Hitomi Sawai, Andrea Pavlou, Yasuo Kato, Yasuhisa Asano, and Shigetoshi Aono
Aldoxime dehydratase: probing the heme environment involved in the synthesis of the carbon
nitrogen triple bond, J. Phys. Chem. B, 115, 13012-13018 (2011).

K. Yasukawa, R. Hasemi, and Y. Asano
Dynamic kinetic resolution of a-aminonitrile to form chiral a-amino acid, Advanced Synthesis & Catalysis, 353 (13), 2328-2332 (2011).

Yasuhisa Asano, Mohammad Dadashipour, Mizue Yamazaki, Nobutaka Doi, and Hidenobu Komeda
Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution: Creation and characterization of highly in vivo soluble mutants, Protein Engineering Design and Selection, 24 (8), 607-616 (2011).

Ken-ichi Fuhshuku, and Yasuhisa Asano
Synthesis of (R)-b-nitro alcohols catalyzed by R-selective hydroxynitrile lyase from Arabidopsis thaliana in the aqueous-organic biphasic system, J. Biotechnol., 153 (3-4), 153-159 (2011).

Mohammad Dadashipour, Mizue Yamazaki, Kazumi Momonoi, Ken'ichirou Tamura, Ken-ichi Fuhshuku, Yurina Kanase, Etsuzoh Uchimura, Guan Kaiyun, Yasuhisa Asano
S-selective hydroxynitrile lyase from a plant Baliospermum montanum: Molecular characterization of recombinant enzyme, J. Biotechnol., 153 (3-4), 100-110 (2011).

Y. Fukuta, S. Nanda, Y. Kato, H. Yurimoto, Y. Sakai, H. Komeda, and Y. Asano
Characterization of a new (R)-hydroxynitrile lyase from the Japanese apricot Prunus mume and cDNA cloning and secretory expression of one of the isozymes in Pichia pastoris, Biosci. Biotech. Biochem., 75 (2), 214-220 (2011).

Mohammad Dadashipour, Yasuhisa Fukuta, and Yasuhisa Asano
Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems, Protein Expr. Purif., 77 (1), 92-97 (2011).

Techawaree Ueatrongchit, and Yasuhisa Asano
Highly selective L-threonine 3-dehydrogenase from Cupriavidus necator and its use in determination of L-threonine, Anal. Biochem., 410 (1), 44-56 (2011).

Mohammad Dadashipour and Yasuhisa Asano
Hydroxynitrile lyases: insights into biochemistry, discovery and engineering, ACS Catalysis, 1 (9), 1121-1149 (2011).

Praveen Kaul and Yasuhisa Asano
Strategies for discovery of novel enzymes: state of the art and opportunities, Microbial Biotechnology, 24 AUG 2011, DOI: 10.1111/j.1751-7915.2011.00280.x

2010

Kimiyasu Isobe, Hiroshi Tamauchi, Ken-ichi Fuhshuku, Shouko Nagasawa and Yasuhisa Asano
A Simple enzymatic method for production of a wide variety of D-amino acids using L-amino acid oxidase from Rhodococcus sp. AIU Z-35-1, Enzyme Research, Article ID 567210 (2010) (online publication).

Motoki Matsuda, and Yasuhisa Asano
Determination of plasma and serum L-lysine using L-lysine e-oxidase from Marinomonas mediterranea NBRC103028^T, Analytical Biochemistry, 406 (1), 19-23 (2010).

I.G. Khaliullin, D.A. Suplatov, D.N. Shalaeva, M. Otsuka, Y. Asano, and V.K. Svedas
Bioinformatic analysis and molecular modeling reveal a role of Lys65 residue in the catalytic triad of D-aminopeptidase from Ochrobactrum anthropi , Acta Naturae (Moscow), 2 (5), 62-66 (2010).

Techawaree Ueatrongchit, Ken'ichirou Tamura, Tohru Ohmiya, Aran H-Kittikun, and Yasuhisa Asano
Hydroxynitrile lyase from Passiflora edulis:Purification, characteristics and application in asymmetric synthesis of (R)-mandelonitrile, Enzyme and Microbial Technology, 46 (6), 456-465 (2010).

Yasuhisa Fukuta, Shinpei Koizumi, Hidenobu Komeda, and Yasuhisa Asano
A new aryl acylamidase from Rhodococcus sp. strain Oct1 acting on w-lactams: its characterization and gene expression in Escherichia coli, Enzyme and Microbial Technology, 46 (3-4), 237-245 (2010).

Yasuhisa Asano
Tools for Enzyme Discovery -"Industrial enzymes, biocatalysis and enzyme evolution"- In "Manual of Industrial Microbiology and Biotechnology" (3rd Edition), Ed.: Richard H. Baltz, Julian E. Davies, and Arnold Demain (American Society for Microbiology) pp 441-452 (2010).

Y. Asano
Phenylalanine Dehydrogenase. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), revised version (2010), in press.

Y. Asano and H. Komeda
D-Aminopeptidase and Alkaline D-peptidase. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), revised version (2010), in press.

Yasuhisa Asano
"Acrylamide", "Inosininc acid", "Guanylic acid" (in Japanese), Dictionary of Biology, (Asakura Press) P10, P96, P334 (2010).

Yasuhisa Asano
Enzymatic synthesis of D-amino acids (in Japanese), "Handbook of enzyme utilization"(NTS Inc.) pp 623-631 (2010).

Yasuhisa Asano
A new (R)-hydroxynitrile lyase from Prunus mume for asymmetric synthesis of cyanohydrins, John Whittall and Peter Sutton (eds.). In Practical Methods in Biocatalysis and Biotransformations, Catalysts for the Fine Chemical Industry, pp 269-272 (2010).

Yasuhisa Asano, Hiroyuki Akita, and Kaoru Nakamura
Selected abstracts from the 12th Japanese Symposium on the Chemistry of Biocatalysis, J. Molec. Catal. B: Enzymatic., 62 (1), 108-126 (2010).

2009

Hitomi Sawai, Hiroshi Sugimoto, Yasuo Kato, Yasuhisa Asano, Yoshitsugu Shiro, and Shigetoshi Aono
X-ray crystal structure of Michaelis complex of aldoxime dehydratase
J. Biol. Chem., 284 (46), 32089-32096 (2009).

I. Kira, Y. Asano and K. Yokozeki
Screening, purification, and identification of the enzyme producing N-(L-a-L-aspartyl)-L-phenylalanine methyl ester from L-isoasparagine and L-phenylalanine methyl ester, J. Biosci. Bioeng., 108 (3), 190-193 (2009).

Yasuhisa Fukuta, Hidenobu Komeda, Yoichi Yoshida, and Yasuhisa Asano
High yield synthesis of 12-aminolauric acid by "Enzymatic transcrystallization" of w-laurolactam using w-laurolactam hydrolase from Acidovorax sp.T31, Biosci. Biotech. Biochem., 73 (5), 980-986 (2009).

Shinjiro Tachibana, Yuko Kuwamori, and Yasuhisa Asano
Discrimination of aliphatic substrates by a single amino acid substitution in Bacillus badius and Bacillus sphaericus phenylalanine dehydrogenases, Biosci. Biotech. Biochem., 73 (3), 729-732 (2009).

Seiji Okazaki, Atsuo Suzuki, Tsunehiro Mizushima, Takeshi Kawano, Hidenobu Komeda, Yasuhisa Asano, and Takashi Yamane
The novel structure of a pyridoxal 5'-phosphate-dependent fold-type I racemase, a-amino-e-caprolactam racemase from Achromobacter obae, Biochemistry, 48 (5), 941-950 (2009).

T. Ueatrongchit, H. Komeda, Y. Asano, and A. H-Kittikun
Parameters influencing asymmetric synthesis of (R)-mandelonitrile by a novel (R)-hydroxynitrile lyase from Eriobotrya japonica, J. Molec. Catal. B: Enzymatic, 56 (4), 208-214 (2009).

Seiji Okazaki, Takashi Yamane, and Yasuhisa Asano
Dynamic kinetic resolution of amino acid amides to form chiral amino acids -structure and function of a-amino-e-caprolactam racemase- (in Japanese), Protein, Nucleic Acids, and Enzyme (Tanpakushitsu, Kakusan, Koso), 54 (11), 1389-1394 (2009).

Yasuhisa Asano
Screening for new functions of enzymes and their industrial development, Bioscience and Industry (in Japanese), 67 (1), 12-18 (2009).

2008

N. Wehofsky, A. Pech, S. Liebscher, S. Schmidt, H. Komeda, Y. Asano, and F. Bordusa
D-amino acid specific proteases and native all-L-proteins: a convenient combination for semisynthesis, Angew. Chem. Int. Ed. Engl., 47 (29) 5456-5460 (2008).

Yasuhisa Asano, Yasuhisa Fukuta, Yoichi Yoshida, and Hidenobu Komeda
The screening, characterization, and use of w-laurolactam hydrolase: A new enzymatic synthesis of 12-aminolauric acid, Biosci. Biotech. Biochem., 72 (8) 2141-2150 (2008).

Hidenobu Komeda and Yasuhisa Asano
A novel D-stereoselective amino acid amidase from Brevibacterium iodinum: Gene cloning, expression and characterization, Enzyme and Microbial Technology, 43 (3), 276-283 (2008).

T. Ueatrongchit, A. Kayo, H. Komeda, Y. Asano, and A. H-Kittikun
Purification and characterization of a novel (R)-hydroxynitrile lyase from Eriobotrya japonica (loquat), Biosci. Biotech. Biochem., 72 (6), 1513-1522 (2008).

Seiji Okazaki, Atsuo Suzuki, Hidenobu Komeda, Yasuhisa Asano, Takashi Yamane
Deduced catalytic mechanism of D-amino acid amidase from Ochrobactrum anthropi SV3, J. Synchrotron Rad, 15, 250-253 (2008).

Seiji Okazaki, Atsuo Suzuki, Tsunehiro Mizushima, Hidenobu Komeda, Yasuhisa Asano, Takashi Yamane
Structures of D-amino acid amidase complexed with L-phenylalanine and with L-phenylalanine amide: Insight into the D-stereospecificity of D-amino acid amidase from Ochrobactrum anthropi SV3, Acta Cryst., D64, 331-334 (2008).

Reynaldo Villalonga, Akira Fujii, Hiroaki Shinohara, Shinjiro Tachibana, and Yasuhisa Asano
Covalent immobilization of phenylalanine dehydrogenase on cellulose membrane for biosensor construction. Sens. Actuators B: Chem., 129, 195-199 (2008).

Koichi Wada, and Yasuhisa Asano
Studies on the microbial production of pyridoxine 5'-a-glucoside (in Japanese), Fine Chemicals, (in Japanese), 37 (1), 36-43 (2008).

Yasuhisa Asano
Enzymatin production of D-amino acids (in Japanese), "Microbial Bioconversion and Bioproduction -Development of White Biotechnology Beyond Chemical Synthesis"(2008), (Supervisor: Mitsuyoshi Ueda, CMC Press), pp 68-77.

Yasuhisa Asano, Hidenobu Komeda, Seiji Okazaki, Takashi Yamane
Discovery of amino acid amidases, and their use in kinetic resolution of amino acid amides, Seikagaku (in Japanese), 80 (4), 294-299 (2008).

Yasuhisa Asano
"Dr. Jyokichi Takamine, an Agricultural Chemist", Foreword to Chemistry and Biology, Chemistry and Biology (in Japanese), 46 (4), 223 (2008).

Yasuhisa Asano
Enzyme handbook, (EC 4.1.2.10, 4.1.2.11, 4.2.1.31, 4.2.1.32, 4.2.1.33, 4.2.1.34, 4.2.1.35, 4.2.1.65, 5.3.3.6) (in Japanese)(Asakura Press)

Yasuhisa Asano and Yasuo Kato
Enzyme handbook, (EC 4.3.1.2) (in Japanese)(Asakura Press)

Yasuhisa Asano and Hidenobu Komeda
Enzyme handbook, (EC 4.2.1.66, 4.2.1.69, 4.2.1.70, 4.2.1.73, 4.2.1.85) (in Japanese)(Asakura Press)

2007

S. Yamaguchi, H. Komeda, and Y. Asano
New enzymatic method of chiral amino acid synthesis by dynamic kinetic resolution of amino acid amides: use of stereoselective amino acid amidases in the presence of a-amino-e-caprolactam racemase, Appl. Environ. Microbiol., 73 (16), 5370-5373 (2007).

Yasuo Kato, Takashi Tsuda, and Yasuhisa Asano
Purification and partial characterization of N-hydroxy-L-phenylalanine decarboxylase/oxidase from Bacillus sp. strain OxB-1, an enzyme involved in aldoxime biosynthesis in the "aldoxime-nitrile pathway". Biochim. Biophys. Acta, 1774 (7), 856-865 (2007).

Seiji Okazaki, Atsuo Suzuki, Hidenobu Komeda, Shigenori Yamaguchi, Yasuhisa Asano, and Takashi Yamane
Crystal structure and functional characterization of a D-stereospecific amino acid amidase from Ochrobactrum anthropi SV3, a new member of the penicillin-recognizing proteins, J. Mol. Biol. 368 (1), 79-91 (2007).

R. Villalonga, A. Fujii, H. Shinohara, Y. Asano, R. Cao, S. Tachibana, and P. Ortiz
Supramolecular-mediated immobilization of L-phenylalanine dehydrogenase on cyclodextrin-coated Au electrodes for biosensor applications, Biotechnol. Lett. 29 (3), 447-452 (2007).

Reynaldo Villalonga, Shinjiro Tachibana, Roberto Cao, Madyu Matos, and Yasuhisa Asano
Glycosidation of phenylalanine dehydrogenase with O-carboxymethyl-poly-b-cyclodextrin, Enzyme Microb. Technol., 40 (3), 471-475 (2007).

Ei-ichiro Suzuki, Kohki Ishikawa, Yasuhiro Mihara, Nobuhisa Shimba, and Yasuhisa Asano
Structural-based engineering for transferases to improve the industrial production of 5'-nucleotide, Bull. Chem. Soc. Jpn., 80 (2), 276-286 (2007).

Yasuhisa Asano, and Sakayu Shimizu
Preface on the special issue of "Directed evolution of biocatalysts and thier uses in industry",Seibutsu-Kogaku Kaishi (in Japanese), 85 (9), 393 (2007)

Yasuhisa Asano, and Mizue Yamazaki
Development of a plant enzyme hydroxynitrile-lyase and its functional expression in Escherichia coli. Seibutsu-Kogaku Kaishi (in Japanese), 85 (9), (2007), 406-408.

Yasuhisa Asano
Creation of methionine dehydrogenase for homocystinuria diagnosis
The collections of Technology-Transfer examples twoards Innovation to improve the quality of life with the "knowledge and wisdom" of universities, in dependent administrative agencies, and technical colleges
Research environment and industrial division, research promotion beaureau, Ministry of Education, Culture, Sports, Science and Technology (MEXT) (in English and Japanese), pp 8-9 (June 2007).

Yasuhisa Asano
Discovery of amino acid racemase activity and its use in the dynamic kinetic resolution of amino acid amides, Fine Chemicals, (in Japanese), 36 (5), 52-59 (2007).

Yasuhisa Asano
Enzymes acting on D-amino acid amides, R. Konno, H. Brueckner, A. D'Aniello, G. Fisher, N. Fujii, and H. Homma (eds.). In D-Amino acids: A new frontier in amino acid and protein research -practical methods and protocols-, Hauppauge, New York, U.S.A., pp 579-589 (2007).

Yasuhisa Asano
Enzymes in aldoxime-nitrile pathway: versatile tools in biocatalysis, Tomoko Matsuda (ed.), In Future directions in bioctalysis, Elsevier, New York, U.S.A., pp 129-139 (2007).

Yasuhisa Asano, Shokichi Ohuchi, and Kohji Ishihara
Selected abstract from the 10th Japanese Symposium on the Chemistry of Biocatalysis, J. Molec. Catal. B: Enzymatic., 48 (3-4), 99-117 (2007).

2006

Katsuaki Kobayashi, Minoru Kubo, Shiro Yoshioka, Teizo Kitagawa, Yasuo Kato, Yasuhisa Asano, and Shigetoshi Aono
Systematic regulation of the enzymatic activity of phenylacetaldoxime dehydratase by exogenous ligands, ChemBioChem., 7 (12), 2004-2009 (2006).

S. Tachibana, M. Suzuki, and Y. Asano
Application of an enzyme chip to the microquantification of L-phenylalanine, Anal. Biochem., 359 (1), 72-78 (2006).

R. Villalonga, S.Tachibana, R. Cao, P.D. Ortiz, L. Gomez, and Y. Asano
Supramolecular-mediated immobilisation of L-phenylalanine dehydrogenase on b-cyclodextrin-modified gold nanospheres, J. Experimental Nanoscience, 1 (2), 249-260 (2006).

K. Kobayashi, B. Pal, S. Yoshioka, Y. Kato, Y. Asano, T. Kitagawa, and S. Aono
Spectroscopic and substrate binding properties of heme-containing aldoxime dehydratases, OxdB and OxdRE, J. Inorganic Biochem., 100 (5-6), 1069-1074 (2006).

Reynaldo Villalonga, Shinjiro Tachibana, Roberto Cao, Hector L. Ramirez, and Yasuhisa Asano
Supramolecular-mediated thermostabilization of phenylalanine dehydrogenase modified with b-cyclodextrin derivatives, Biochem. Eng. J., 30 (1), 26-32 (2006).

Samik Nanda, Yasuo Kato, and Yasuhisa Asano
PmHNL catalyzed synthesis of (R)-cyanohydrins derived from aliphatic aldehydes, Tetrahedron: Asymmetry, 17 (5), 735-741 (2006).

H. Komeda, N. Hariyama and Y. Asano
L-Stereoselective amino acid amidase with broad substrate specificity from Brevundimonas diminuta: characterization of a new member of the leucine aminopeptidase family, Appl. Microbiol. Biotechnol., 70 (4), 412-421 (2006).

Y. Kato and Y. Asano
Molecular and enzymatic analysis of the "aldoxime-nitrile pathway" in the glutaronitrile degrader Pseudomonas sp. K-9, Appl. Microbiol. Biotechnol., 70 (1), 92-101 (2006).

Yasuhisa Asano, Hideo Hirohara, and Kohji Ishihara
Selected abstracts from the 9th Japanese Symposium on the Chemistry of Biocatalysis, J. Molec. Catal. B: Enzymatic., 42 (3-4), 114-135 (2006).

2005

J. Sharma, D. Batovska, Y. Kuwamori, and Y. Asano
Enzymatic chemo selective synthesis of secondary-amide surfactant from N-methylethanol amine, J. Biosci. Bioeng., 100 (6), 662-666 (2005).

Y. Asano, K. Tamura, N. Doi, T. Ueatrongchit, A. H-Kittikun, and T. Ohmiya
Screening for new hydroxynitrilases from plants, Biosci. Biotech. Biochem., 69 (12), 2349-2357 (2005).

Y. Kato, and Y. Asano
Purification and characterization of aldoxime dehydratase of the head blight fungus, Fusarium graminearum, Biosci. Biotech. Biochem., 69 (11), 2254-2257 (2005).

S. Nanda, Y. Kato, and Y. Asano
A new (R)-hydroxynitrile lyase from Prunus mume: asymmetric synthesis of cyanohydrins, Tetrahedron, 61 (46), 10908-10916 (2005).

Y. Asano and S. Yamaguchi
Discovery of amino acid amides as new substrates for a-amino-e-caprolactam racemase from Achromobacter obae, J. Molec. Catal. B: Enzymatic., 36 (1-6), 22-29 (2005).

R. Villalonga, S. Tachibana, Y. Perez, and Y. Asano
Increased conformational and thermal stability properties for phenylalanine dehydrogenase by chemical glycosidation with end-group activated dextran, Biotechnol. Lett., 27 (17), 1311-1317 (2005).

H. Komeda and Y. Asano
A DmpA-homologous protein from Pseudomonas sp. is a dipeptidase specific for b-alanyl dipeptides. FEBS J., 272, 3075-3084 (2005).

A. Inoue, H. Komeda, and Y. Asano
Asymmetric synthesis of L-a-methylcysteine with the amidase from Xanthobacter flavus NR303, Advanced Synthesis & Catalysis, 347, 1132-1138 (2005).

T. Hongpattarakere, H. Komeda, and Y. Asano
Purification, characterization, gene cloning and nucleotide sequencing of D-stereospecific amino acid amidase from soil bacterium: Delftia acidovorans, J. Industrial Microbiol. Biotechnol., 32 (6), 567-576 (2005).

Y. Asano and S. Yamaguchi
Dynamic kinetic resolution of amino acid amide catalyzed by D-aminopeptidase and a-amino-e-caprolactam racemase, J. Am. Chem. Soc., 127 (21), 7696-7697 (2005).

Y. Kato, S. Yoshida, and Y. Asano
Polymerase chain reaction for identification of aldoxime dehydratase in aldoxime- or nitrile-degrading microorganisms, FEMS Microbiol. Lett., 246 (2), 243-249 (2005).

Y. Asano, I. Kira, and K. Yokozeki
Alteration of substrate specificity of aspartase by directed evolution, Biomolecular Engineering, 22 (1-3), 95-101 (2005).

K. Kobayashi, S. Yoshioka, Y. Kato, Y. Asano, and S. Aono
Regulation of Aldoxime Dehydratase Activity by Redox-dependent Change in the Coordination Structure of the Aldoxime-Heme Complex, J. Biol. Chem., 280 (7), 5486-5490 (2005).

Y. Asano
Enzymatic synthesis of (S)-phenylalanine and related (S)-amino acids by phenylalanine dehydrogenase, Microbial Enzymes and Biotransformations, J. L. Barredo, (ed.). In Methods in Biotechnology, Humana Press (2005), pp 157-166.

Y. Asano
Development of D-stereoselective amino acid amido hydrolases and their application to the dynamic kinetic resolution of amino acid amides. Pharmacia, (in Japanese), 41 (9), 881-884 (2005).

Y. Asano
Establishment of the Department of Biotechnology, Toyama Prefectural University and "Green Biotechnology" (in Japanese), In Toyama Monthly Economics, 8, (2005), published online.

S. Okazaki, T. Hikage, A. Suzuki, H. Komeda, Y. Asano, and T. Yamane
Structure determination of D-stereospecific amino acid amidase (in Japanese), In Progress in X-ray diffraction reseach, Nagoya University, Japan (2005), 26, pp 21-23

H. Komeda, N. Mizuguchi, I. Kira, K. Yokozeki, and Y. Asano
Cloning of isoasparaginase (a-aspartyl dipeptidase) gene from Escherichia coli K-12 and overexpression of the gene in E. coli. Bull. Toyama Pref. Univ., 15, 91-96 (2005).

Y. Asano, H. Ohta, and K. Ishihara
Selected abstracts from the 8th Japanese symposium on the chemistry of biocatalysis. J. Molec. Catal. B: Enzymatic., 36 (1-6), 54-74 (2005).

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2004

Y. Mihara, K. Ishikawa, E. Suzuki, and Y. Asano
Improving the pyrophosphate-inosine phosphotransferase activity of Escherichia blattae acid phosphatase by sequential site-directed mutagenesis, Biosci. Biotechnol. Biochem., 68 (5), 1046-1050 (2004).

D. I. Batovska, S. Tsubota, Y. Kato, Y. Asano, and M. Ubukata
Lipase-mediated desymmetrization of glycerol with aromatic and aliphatic anhydrides, Tetrahedon Asymmetry, 15 (22), 3551-3559 (2004).

Y. Asano, Y. Kato, C. Levy, P. Baker, and D. Rice
Structure and function of amino acid ammonia-lyases, Biocatal. Biotransform., 22 (2), 131-138 (2004).

H. Komeda, H. Harada, S. Washika, T. Sakamoto, M. Ueda, and Y. Asano
A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide, Eur. J. Biochem., 271 (8), 1580-1590 (2004).

H. Komeda, H. Harada, S. Washika, T. Sakamoto, M. Ueda, and Y. Asano
S-Stereoselective piperazine-2-tert-butylcarboxamide hydrolase from Pseudomonas azotoformans IAM 1603 is a novel L-amino-acid amidase, Eur. J. Biochem., 271 (8), 1465-1475 (2004).

Y. Kato, S. Yoshida, S.-X. Xie, and Y. Asano
Aldoxime dehydratase co-existing with nitrile hydratase and amidase in the iron-type nitrile hydratase-producer Rhodococcus sp. N-771, J. Biosci. Bioeng., 97 (4), 250-259 (2004).

Y. Asano, N. Mizuguchi, H. Komeda, I. Kira, and K. Yokozeki
Identification of Isoasparaginase from Escherichia coli K-12 as a-aspartyl dipeptidase, Bull. Toyama Pref. Univ., 14, 96-103 (2004).

H. Komeda and Y. Asano
Functions of the D-stereoselective peptidases and their applications (in Japanese), Bioscience and Industry., 62 (9), 591-594 (2004).

H. Komeda and Y. Asano
Screening for the stereoselective amino acid amidsaes and their use for the synthesis of optically active amino acids (in Japanese), Seibutsu Kougaku Kaishi, 82 (6), 237-240 (2004).

Y. Asano and H. Kaga
Selected Abstracts from the 7th Japanese Symposium on the Chemistry of Biocatalysis. J. Molec. Catal. B: Enzymatic, 30(5-6), 241-256 (2004).

2003

S. Y. K. Seah, K. L. Britton, D. W. Rice, Y. Asano, P. C. Engel
Kinetic analysis of phenylalanine dehydrogenase mutants designed for aliphatic amino acid dehydrogenase activity with guidance from homology-based modelling, Eur. J. Biochem., 270 (23), 4628-4634 (2003).

K. Wada and Y. Asano
Use of borate to control the 5'-position selective microbial glucosylation of pyridoxine, Appl. Environ. Microbiol., 69 (12), 7058-7062 (2003).

H. Komeda and Y. Asano
Genes for an alkaline D-stereospecific endopeptidase and its homolog are located in tandem on Bacillus cereus genome, FEMS Microbiol. Lett., 228 (1), 1-9 (2003).

S.-X. Xie, Y. Kato, H. Komeda, S. Yoshida, and Y. Asano
A gene cluster responsible for alkylaldoxime metabolism coexisting with nitrile hydratase and amidase in Rhodococcus globerulus A-4, Biochemistry, 42 (41), 12056-12066 (2003).

Y. Kato and Y. Asano
High-level expression of a novel FMN-dependent heme-containing lyase, phenylacetaldoxime dehydratase of Bacillussp. Strain OxB-1, in heterologous hosts, Protein Expr. Purif., 28 (1), 131-139 (2003).

D. H. Baek, J. J. Song, S.-G. Lee, S. J. Kwon, Y. Asano, and M.-H. Sung
New thermostable D-methionine amidase from Brevibacillus borstelensis BCS-1 and its application for D-phenylalanine production, Enzyme Microb. Technol., 32 (1), 131-139 (2003).

K. Wada and Y. Asano
Improvement in 5'-position selective glucosylation of pyridoxine by Verticillium dahliae TPU4900, Biosci. Biotech. Biochem., 67 (3), 508-516 (2003).

Y. Asano and K. Wada
Regioselective glucosylation of pyridoxine by microorganisms, Biosci. Biotech. Biochem., 67 (3), 499-507 (2003).

H. Komeda, N. Ishikawa, and Y. Asano
Enhancement of the thermostability and catalytic activity of D-stereospecific amino-acid amidase from Ochrobactrum anthropi SV3 by directed evolution, J. Molec. Catal. B: Enzymatic., 21 (4-6), 283-290 (2003).

Y. Asano
Two solutions in the structure and function of amino acid ammonia-lyases, Seibutsu-Kogaku Kaishi (in Japanese), 81 (5), 193 (2003).

Y. Asano
Chorismate mutase , In "Protein Chemistry" (in Japanese), T. Fukui and M. Itoh Eds., Hirokawa Press, vol 4, pp. 181-185 (2003), ISBN4-567-16047-9.

Y. Asano
Structure and function of amino acid ammonia-lyases, Kagaku to Kogyo (Osaka) (in Japanese), 77 (2), 71-78 (2003).

Y. Asano
Let soil microorganisms work!, Kagaku-Kogyo (Chemical Industry) (in Japanese), 54 (2), 124-127 (2003).

Y. Asano and K. Nakamura
Selected Abstracts from the 6th Japanese Symposium on the Chemistry of Biocatalysis. J. Molec. Catal. B: Enzymatic, 22 (3-4), 225-250 (2003).

2002

S. Y. K. Seah, K. L. Britton, D. W. Rice, Y. Asano, and P. C. Engel
Single amino acid substitution in Bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine substrates, Biochemistry, 41 (38), 11390-11397 (2002).

C. W. Levy, P. A. Buckley, S. Sedelnikova, Y. Kato, Y. Asano, D. W. Rice, and P. J. Baker
Insights into enzyme evolution revealed by the structure of methylaspartate ammonia lyase, Structure, 10, 105-113 (2002).

K. Ishikawa, Y. Mihara, N. Shimba, N. Ohtsu, H. Kawasaki, E. Suzuki, and Y. Asano
Enhancement of nucleoside phosphorylation activity in an acid phosphatase, Protein Engineering, 15 (7), 539-543 (2002).

E. Omidinia, H. Taherkhani, Y. Asano, S. Khathami, A. Omumi, A.-A. Ghadiri, D. van der Lelie, R. Rashid Pouraie, H. Mirzahoseini, and A. Samadi
Affinity purification and characterization of recombinant Bacillus sphaericus phenylalanine dehydrogenase produced by pET expression vector system. Iranian Biomedical Journal, 6 (1), 31-36 (2002).

E. Omidinia, A. Samadi, H. Taherkhani, S. Khatami, N. Moazami, R. Rashid Pouraie, and Y. Asano
Cloning and expression of Bacillus sphaericusphenylalanine dehydrogenase gene in Bacillus subtiliscells: purification and enzyme properties, World J. Microbiol. Biotechnol., 18(7), 593-597 (2002).

H. Komeda and Y. Asano
Discovery of D-stereospecific peptidases of microbial origin: what are their roles? (in Japanese) Chemistry and Biology (Kagaku to Seibutsu, Gakkai Syuppan Center Press), 40 (7), 429-431 (2002).

Y. Kato and Y. Asano
New insights for amino acid ammonia-lyases (in Japanese) Bioscience and Industry (in Japanese) 60(9), 32-33 (2002).

Y. Asano
Screening for microbial enzymes and thier fine tuning to application -Phosphorylation of nucloside by mutated acid phosphatase-, The Hokuriku Journal of Radioisotope Research (in Japanese), 4, 46-47 (2002).

Y. Asano and Y. Kato
Screening for industrial enzymes and fine tuning of thier activities, In "Green Biotechnology -Biotechnolgy for sustainable society" (in Japanese), H. Un'no and Y. Okahata Eds., Kodan-sya Press, pp. 71-82 (2002), ISBN4-06-154310-5.

Y. Asano
Overview of screening for new microbial catalysts and their uses in organic synthesis -Selection and optimization of biocatalysts-, J. Biotechnol., 94 (1), 65-72 (2002).

Y. Asano and T. Ito
Selected Abstracts from the 5th Japanese Symposium on the Chemistry of Biocatalysis. J. Molec. Catal. B: Enzymatic., 18(1-3), 173-197 (2002).

2001

E. Omidinia, F. Mahboundi, S. Khatami, D. van der Lelie, N. Moazami, Y. Asano, and A. Samadi
Molecular cloning and evaluation of wild promoter in expression of Bacillus sphaericus phenylalanine Dehydrogenase gene in Bacillus subtilis cells. Journal of Sciences (Iranian Journal) (Tehran, Iran), 12 (4), 307-313 (2001).

C. W. Levy, P. A. Buckley, P. J. Baker, S. Sedelnikova, F. Rodgers, Y.-F. Li, Y. Kato, Y. Asano, and D. W. Rice
Crystallization and preliminary X-ray analysis of Citrobacter amalonaticus methylaspartate ammonia Lyase, Acta Cryst. D., D57, 1922-1924 (2001).

S.-X. Xie, Y. Kato, and Y. Asano
High yield synthesis of nitriles by a new enzyme, phenylacetaldoxime dehydratase, from Bacillus sp. strain OxB-1, Biosci. Biotech. Biochem., 65 (12), 2666-2672 (2001).

Y. Mihara, T. Utagawa, H. Yamada, and Y. Asano
Acid phosphatase/phosphotransferases from enteric bacteria, J. Biosci. Bioeng., 92 (1), 50-54 (2001).

J.-H. Xu, Y. Kato and Y. Asano
Efficient preparation of (R)-a-Monobenzoyl glycerol by lipase catalyzed asymmetric esterification: optimization and operation in packed bed reactor, Biotechnol. Bioeng., 73 (6), 493-499 (2001).

Y. Kato and Y. Asano
A new enzymatic method of stereoselective oxidation of racemic 1,2-indandiols, J. Molec. Catal. B: Enzymatic., 13 (1-3), 27-36 (2001).

Y. Asano and S. Watanabe
Isolation of poly(3-Hydroxybutyrate) (PHB)-degrading microorganisms and characterization of PHB-depolymerase from Arthrobacter sp. strain W6, Biosci. Biotech. Biochem.,65 (5), 1191-1194 (2001).

Y. Kato and Y. Asano
Occurrence of a novel lyase catalyzing b-elimination reaction toward threo-3-chloro-L-aspartate in Pseudomonas putida TPU 7151, Biosci. Biotech. Biochem.,65 (2), 435-437 (2001).

Y. Asano, M. Umezaki, Y.-F. Li, S. Tsubota, T. L. Luebbehuesen
Isolation of microorganisms which utilize acidic D-amino acid oligomers. J. Molec. Catal. B: Enzymatic, 12 (1-6), 53-59 (2001).

Y. Asano
Biotechnology 2000 (in Japanese), Enzyme Engineering News, 45, 45-46 (2001).

Y. Asano
Selected abstracts from the 3rd Japanese symposium on the chemistry of biocatalysis. J. Molec. Catal. B: Enzymatic., 14 (1-3), 45-66 (2001).

Preface to a special issue "D-Amino acid bioscience and biotechnology." J. Molec. Catal. B: Enzymatic, 12, 1 (2001).

2000

K. Ishikawa, Y. Mihara, K. Gondoh, E. Suzuki, and Y. Asano
X-ray structures of a novel acid phosphatase from Escherichia blattae and its complex with the transition-state analog molybdate, EMBO J.,19 (11), 2412-2423 (2000).

Y. Mihara, T. Utagawa, H. Yamada, and Y. Asano
Phosphorylation of nucleosides by the mutated acid phosphatase from Morganella morganii, Appl. Environ. Microbiol., 66 (7), 2811-2816 (2000).

Y. Kato, R. Ooi, and Y. Asano
Distribution of aldoxime dehydratase in microorganisms, Appl. Environ. Microbiol., 66 (6), 2290-2296 (2000).

H. Komeda and Y. Asano
Gene cloning, Nucleotide sequencing, and purification and characterization of the D-stereospecific amino-acid amidase from Ochrobactrum anthropi SV3., Eur. J. Biochem., 267, 2028-2035 (2000).

Y. Kato, I. Fujiwara, and Y. Asano
Synthesis of optically active a-monobenzoyl glycerol by asymmetric transesterification of glycerol. J. Molec. Catal. B: Enzymatic, 9, 193-200 (2000).

Y. Kato, K. Nakamura, H. Sakiyama, S. G. Mayhew, and Y. Asano
Novel heme-containing lyase, phenylacetaldoxime dehydratase from Bacillus sp. strain OxB-1: purification, characterization, and molecular cloning of the gene. Biochemistry, 39, 800-809 (2000).

Y. Asano and T. Luebbehuesen
Enzymes acting on peptides containing D-amino acid. J. Bioscience and Bioengineering, 89 (4), 295-306 (2000).

Y. Asano
Selection and directed evolution of new microbial biocatalysts and their application to organic synthesis, Agric. Chem. Biotechnol., 43 (4), 207-210 (2000).

Y. Asano
New enzymes acting on peptides containing D-amino acids: thier properties and application, J. Microbiol. Biotechnol., 10 (5), 573-579 (2000).

H. Komeda, H. Harada, Y. Asano
Screening for 4,6-bisphenylazoresorcinol (4,6-Bis) degrading microorganisms (in Japanese). Bull. Toyama Pref. Univ., 10, 96-102 (2000).

Y. Mihara and Y. Asano
Phosphorylation of nucleosides by mutated acid phosphatase, Bioscience and Industry (in Japanese), 58 (12), 865-866(2000).

Y. Asano
Enzymes in Biotechnology (in Japanese). J. Phys. Chem. Earth Sci. (Toyama), 42, 3-6 (2000).

Preface to a special issue "Professor Saburo Fukui and his scientific achievement." J. Molec. Catal. B: Enzymatic, 10, 1-3 (2000).

1999

Y. Kato, I. Fujiwara, and Y. Asano
A novel method for preparation of optically active a-monobenzoyl glycerol via lipase-catalyzed asymmetric transesterification of glycerol., Bioorg. Med. Chem. Lett., 9, 3207-3210 (1999).

Y. Asano, Y. Mihara, and H. Yamada
A novel selective nucleoside phosphorylating enzyme from Morganella morganii. J. Biosci. Bioeng., 87 (6), 732-738 (1999).

Y. Kato, T. Tsuda, and Y. Asano
Nitrile hydratase involved in aldoxime metabolism from Rhodococcus sp. strain YH3-3: purification and characterization. Eur. J. Biochem., 263, 662-670 (1999).

Y. Kato, R. Ooi, and Y. Asano
A New Enzymatic Method of Nitrile Synthesis by Rhodococcus sp. strain YH3-3. J. Molec. Catal. B: Enzymatic, 6, 249-256 (1999).

Y. Asano, Y. Mihara, and H. Yamada
A New Enzymatic Method of Selective Phosphorylation of Nucleosides. J. Molec. Catal. B: Enzymatic, 6, 271-277 (1999).

H. Komeda and Y. Asano
Synthesis of D-Phenylalanine Oligopeptides Catalyzed by Alkaline D-Peptidase from Bacillus cereus DF4-B. J. Molec. Catal. B: Enzymatic, 6, 379-386 (1999).

H. Kawashima, S. Mitamura, Y. Kato, and Y. Asano
Microbial Production of Optical Active Indene Derivatives (In Japanese), Aromatics (Tokyo), 51 (9,10), 319-325 (1999).

Y. Asano and H. Komeda
D-Aminopeptidase and Alkaline D-peptidase. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), p.111-121 (1999).

Y. Asano
Phenylalanine Dehydrogenase.Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), p.1955-1963 (1999).

Y. Kato and Y. Asano
Opine Dehydrogenase and Secondary Amine Dicarboxylic Acids. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), p.1851-1858 (1999).

M. Ueda, H. Yamada, and Y. Asano
D-Malate. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), p.1579-1585 (1999).

Y. Asano
Selected Abstracts from the 2nd Japanese Symposium on the Chemistry of Biocatalysis. J. Molec. Catal. B: Enzymatic., 7, 311-345 (1999).

Y. Asano
Microbial aldoxime metabolism and the enzymatic synthesis of nitriles. Bioscience and Industry (in Japanese) 57, 251-252 (1999).

Y. Asano
Development of new microbial enzymes and their application to organic synthesis. Jap. J. Syn. Org. Chem. (in Japanese), 57, 1064-1072 (1999).

1998

K.L. Britton, H.F. Rogers, Y. Asano, T. Dairi, Y. Kato, T.J. Stillman, and D.W. Rice
Crystallization of Arthrobacter sp. strain 1C N-(1-D-carboxyethyl)-L-norvaline dehydrogenase and its complex with NAD⁺. Acta Cryst. D. 54, 124-126 (1998).

K.L. Britton, Y. Asano, and D.W.Rice
Crystal structure and active site location of N-(1-D-carboxylethyl)-L-norvaline dehydrogenase. Nature Structural Biology, 5 (7), 593-601 (1998).

Y. Asano and M. Tanetani
Thermostable phenylalanine dehydrogenase from a mesophilic Microbacterium sp. strain DM86-1, Arch. Microbiol., 169, 220-224 (1998).

Y. Asano and Y. Kato
Z-Phenylacetaldoxime degradation by a novel aldoxime dehydratase from Bacillus sp. strain OxB-1. FEMS Microbiol. Lett., 158, 185-190 (1998).

Y. Kato, R. Ooi, and Y. Asano
Isolation and characterization of a bacterium possessing a novel aldoxime- dehydration activity and nitrile-degrading enzymes. Arch. Microbiol., 170, 85-90 (1998)

Y. Kato, and Y. Asano
Cloning, Nucleotide Sequencing, and Expression of the 3-Methylaspartate Ammonia Lyase Gene from Citrobacter amalonaticus strain YG-1002. Appl. Microbiol. Biotechnol., 50, 468-474 (1998)

Y. Asano
A Japanese Screening Approach -Selection of an opine Dehydrogenase and Alkaline D-Peptidase-. Studies in Organic Chemistry 53, Proceedings of "New Frontiers in screening for microbial biocatalysts" K. Kieslich, C.P. van der Beek, J.A.M. de Bont and W.J.J. van den Tweel, Ed., Elsevier (1998) 19-28.

Y. Asano
D-Stereospecific aminopeptidase. Handbook of Proteolytic Enzymes (Academic Press), 430-433 (1998).

Y. Asano
Alkaline D-peptidase. Handbook of Proteolytic Enzymes (Academic Press), 433-435 (1998).

Y. Asano
Selected Abstracts from the 1st Japanese Symposium on the Chemistry of Biocatalysis. Journal of Molecular Catalysis B: Enzymatic., 5, 491-513 (1998).

Y. Asano and Y. Kato
Discovery of a new enzyme opine dehydrogenase and its application. Seibutsu Kogaku Kaishi, (in Japanese) 76, 426-429 (1998).

1997

Y. Kato and Y. Asano
3-Methylaspartate Ammonia-Lyase as a Marker Enzyme of the Mesaconate Pathway for (S)-Glutamate Fermentation in Enterobacteriaceae. Arch. Microbiol., 168, 457-463 (1997).

1996

Ivo Frebort, Hisanori Tamaki, Hiroki Ishida, Pavel Pec, Lenka Luhova, Hiroko Tsuno, Martin Halata, Yasuhisa Asano, Yasuo Kato, Kazunobu Matsushita, Hirohide Toyama, Hidehiko Kumagai and Osao Adachi
Two distinct quinoprotein amine oxidases are induced by n-butylamine in the mycelia of Aspergillus niger AKU 3302. purification, characterization, cDNA cloning and sequencing. Eur. J. Biochem., 237, 255-265 (1996).

Ivo Frebort, Pavel Pec, Lenka Luhova, Hirohide Toyama, Kazunobu Matsushita, Shun Hirota, Teizo Kitagawa, Tamio Ueno, Yasuhisa Asano, Yasuo Kato, and Osao Adachi
Two amine oxidases from Aspergillus niger AKU 3302 contain topa quinone as the cofactor: unusual cofactor link to the glutamyl residue occurs only at one of the enzymes. Biochim. Biophys. Acta, 1295, 59-72 (1996).

K. Nakamura, T. Fujii, Y. Kato, Y. Asano and A. J. L. Cooper
Quantitation of L-amino acids by substrate recycling between an aminotransferase and a dehydrogenase: application to the determination of L-phenylalanine in human blood. Anal. Biochem., 234, 19-22 (1996).

Y. Kato, H. Yamada and Y. Asano
Stereoselective synthesis of opine-type secondary amine carboxylic acids by a new enzyme opine dehydrogenase use of recombinant enzymes-. J. Molec. Catal. B: Enzymatic, 1, 151-160 (1996).

Y. Kato, Y. Asano, and A. J. L. Cooper
Inactivation of brain and kidney aspartate aminotransferases by S-(1,2-dichlorovinyl)-L-cysteine and by S-(1,1,2,2-tetrafluoroethyl)-L-cysteine. Developmental Neuroscience, 18, 505-514 (1996).

Y. Kato, Y. Asano, Tapas K. Makar, and A. J. L. Cooper
Irreversible inactivation of aspartate aminotransferase by 2-oxoglutaconic acid and its dimethyl ester. J. Biochem. (Tokyo), 120 (3), 531-539 (1996).

Y. Asano, H. Ito, T. Dairi, and Y. Kato
An alkaline D-stereospecific endopeptidase with b-lactamase activity from Bacillus cereus. J. Biol. Chem., 271 (47), 30256-30262 (1996).

Y. Asano, M. Tanetani and N. Horita
Screening for novel amino acid dehydrogenases -Soil of Toyama Prefecture as a source of microorganisms- (in Japanese). Bull. Toyama Pref. Univ., 6, 101-106 (1996).

Y. Asano
Toyama Prefectural University (in Japanese), Chubu Bio Forum 10, 20-23 (1996).

Y. Asano
The second Japanese-Dutch workshop on biocatalysis (in Japanese), Enzyme Engineering News, 35, 34-35 (1996).

Y. Asano
Liquefaction of coal by enzymes and microorganisms (in Japanese), Chemistry and Industry, 49, 954 (1996).

1995

Y. Kato and Y. Asano
Purification and properties of crystalline 3-methylaspartase ammonia-lyase from two facultative anaerobes, Citrobacter sp. Strain YG-0504 and Morganella morganii strain YG-0601. Biosci. Biotech. Biochem., 59 (1), 93-99 (1995).

Y. Asano and K. Yamaguchi
Mutants of D-aminopeptidase with increased thermal stability. J. Ferment. Bioeng., 79 (6), 614-616 (1995).

Y. Kato, Y. Asano and A. J. L. Cooper
3-Hydroxy-2-oxo-1-oxaspiro[4.5]-dec-3-ene, a new inhibitor of lactate dehydrogenase. Tetrahedron Letters, 36 (27), 4809-4812 (1995).

T. Dairi and Y. Asano
Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C. Appl. Environ. Microbiol., 61 (8), 3169-3171 (1995).

S. Y.K. Seah, K. L. Britton, P. J. Baker, D. W. Rice, Y. Asano and P. C. Engel
Alteration in relative activities of phenylalanine dehydrogenase towards different substrates by site-directed mutagenesis. FEBS Letters, 370, 93-96 (1995).

A. Yamada, T. Dairi, Y.Ohno, X.-L. Huang and Y. Aasano
Nucleotide Sequencing of phenylalanine dehydrogenase gene from Bacillus badius IAM 11059. Biosci. Biotech. Biochem., 59 (10), 1994-1995 (1995).

Y. Kato and Y. Asano
3-Methylaspartate ammonia-lyase from a facultative anaerobe, strain YG-1002. Appl. Microbiol. Biotechnol., 43, 901-907 (1995).

M. A. Smal, Z. Dong, H. T. A. Cheung, Y. Asano, L. Escoffier, M. Costello and M. H. N. Tattersall
Activation and cytotoxicity of 2-a-aminoacyl prodrugs of methotrexate. Biochem. Pharmacol., 49 (4), 567-574 (1995).

H. Inoue, A. Takano, Y. Asano and Y. Katoh
Purification and Characterization of a 22-kDa protein in chloroplasts from green spores of the fern Osmunda japonica. Physiol. Plant., 95, 465-471 (1995).

Y. Kato, J. Yamagishi and Y. Asano
Maleate cis-trans isomerase from Arthrobacter sp. TPU 5446. J. Ferment. Bioeng., 80 (6), 610-612 (1995).

Y. Kato, S. Ooe and Y. Asano
Screening for Novel Ammonia-lyases (in Japanese). Bull. Toyama Pref. Univ., 5, 114-126 (1995).

1994

M. Ueda, H. Yamada and Y. Asano
The Substrate Specificity of maleate hydratase from Arthrobacter sp. strain MCI2612. Appl. Microbiol. Biotechnol., 41, 215-218 (1994).

Y. Asano and Y. Kato
Crystalline 3-methylaspartase from a facultative anaerobe,Escherichia coli strain YG1002. FEMS Microbiol. Lett., 118, 255-258 (1994).

Y. Asano and Y. Kato
Occurrence of 3-Methylaspartate ammonia-lyase in facultative anaerobes and their application to synthesis of 3-substituted (S)-aspartic acids. Biosci. Biotech. Biochem., 58 (1), 223-224 (1994).

Xin-Li Huang and Y. Asano
Screening for novel phenylalanine dehydrogenases (in Japanese). Bull. Toyama Pref. Univ., 4, 211-215 (1994).

M. Ohto, A. Yamamoto, A. Matsunaga, Y. Asano, Y, Kawata, and E. Mizukami
Structure of copper dhlorophyll analogues in the chewing gum product (in Japanese), Jpn. J. Toxicol. Environ. Health, 40, 279-285 (1994).

Y. Asano, Y. Yamamoto, and H. Yamada
Catechol-2,3-dioxygenase-catalyzed synthesis of picolinic acids from catechols. Biosci. Biotech. Biochem., 58 (11), 2054-2056 (1994).

Y. Asano, T. Komeda and H. Yamada
Enzymes Involved in theobromine production from caffeine by Pseudomonas putida No.352. Biosci. Biotech. Biochem., 58 (12), 2303-2304 (1994).

Y. Asano
Biotechnology and agricultural chemistry, Nippon Nogei Kagaku Kaishi, 68, 1416-1417 (1994).

Y. Asano
Encyclopedia of Toyama (in Japanese), biotechnology, vitamin, gene manipulation, fermentation, enzyme, tissue culture, cell fusion, Kitanihon Shinbun (1994).

Y. Asano
Utilization of the functions of organisms (in Japanese), Science and Technology in Toyama (Toyama Prefecture), 12-17 (1994).

Y. Asano
Letting microorganisms synthesize useful materials (in Japanese), Science and Technology in Toyama (Toyama Prefecture), 118-119 (1994).

1993

Y. Asano, M. Ueda and H. Yamada
Microbial production of D-malate from maleate. Appl. Environ. Microbiol., 59 (4), 1110-1113 (1993).

Y. Kato, K. Fukumoto and Y. Asano
Enzymatic synthesis of L-b-chloroalanine using amino acid dehydrogenase. Appl. Microbiol. Biotechnol., 39, 301-304 (1993).

Y. Asano, T. Komeda and H. Yamada
Microbial production of theobromine from caffeine. Biosci. Biotech. Biochem., 57 (8), 1286-1289 (1993).

M. Ueda, Y. Asano and H. Yamada
Purification and Characterization of Maleate Hydratase from Arthrobacter sp. strain MCI2612. Biosci. Biotech. Biochem., 57 (9), 1545-1548 (1993).

M. Ueda, R. Sashida, H. Yamada and Y. Asano
Production of S-(+)-citramalic acid from itaconic acid by resting cells of Alcaligenes denitrificans strain MCI2775. Appl. Microbiol. Biotechnol., 40, 466-469 (1993).

Y. Asano
The first Japanese-Dutch workshop on biocatalysis (in Japanese), Bioscience and Bioindustry, 51, 911-912 (1993).

1992

Y. Asano, Y. Kato, A. Yamada and K. Kondo
Structural similarity of D-aminopeptidase to carboxypeptidase DD and b-Lactamase. Biochemistry, 31, 2316-2328 (1992).

Y. Asano
A new enzyme D-Aminopeptidase: structure, function, and application to organic synthesis. Frontiers and New Horizons in Amino Acid Research, K. Takai Ed. (1992) 333-336, Elsevier

Y. Asano
Syntheses of amino acids by new microbial enzymes (in Japanese), Nippon Nogei Kagaku Kaishi, 66, 1117-1118 (1992).

Y. Asano, M. Ono, T. Sato, H. Nagaoka, J. Matsuda, S. Ogawa and T. Takeda
Current status and future perspective of synthetic organic chemistry (in Japanese), Japanese Journal of Synthetic Organic Chemistry, 50, 1116-1124 (1992) .

1991

Y. Asano, K. Kishino, A. Yamada, S. Hanamoto and K. Kondo
Plasmid-based, D-aminopeptidase-catalysed synthesis of (R)-amino acids. Recl. Trav. Chim. Pays-Bas, 110, 206-208 (1991).

B. R. Blanco-De Guzman, Y. Asano and H. Yamada
Microbial degradation of opines: isolation and characterization of an opine utilizing bacterium. Annual Reports of International Center of Cooperative Research and Development in Microbial Engineering, Japan, 14, 167-178 (1991).

Y. Asano
A new enzyme D-aminopeptidase -Structure, Its function and application to organic synthesis (in Japanese), Japanese Journal of Synthetic Organic Chemistry, 49, 314-326 (1991).

Y. Asano
Strecker amino acid synthesis (in Japanese), Japanese Journal of Synthetic Organic Chemistry, 49, 350 (1991).

Y. Asano
Enrich culture and acclimation techniques (in Japanese), Japanese Journal of Synthetic Organic Chemistry, 49, 350 (1991).

Y. Asano
Discovery of a new enzyme D-aminopeptidase and its application (in Japanese), Bioindustry, 8, 338-345 (1991).

Y. Asano
Discovery of a new enzyme D-aminopeptidase and its application to organic synthesis (in Japanese), Enzyme Engineering News, 25, 11-14 (1991).

Y. Asano
Syntheses of useful amides and amino acids by the use of new microbial enzymes (in Japanese), Nippon Nogei Kagaku Kaishi, 65, 710-712 (1991).

Y. Asano
Syntheses of useful amides and amino acids by the use of new microbial enzymes (in Japanese), Nippon Nogei Kagaku Kaishi, 65, 1617-1626 (1991).

1990

A. S. Gelbard, A. J. L. Cooper. Y. Asano, E. Nieves, S. Filc-DeRico and K. C. Rosenspire
Methods for the Enzymatic synthesis of tyrosine and phenylalanine labeled with nitrogen-13. Appl. Rad. Isotopes, 41 (2), 229-233 (1990).

Y. Kato, Y. Asano, A. Nakazawa and K. Kondo
Synthesis of D-alanine oligopeptides by D-aminopeptidase in non-aqueous Media. Biocatalysis, 3, 207-215 (1990).

Y. Asano, A. Yamada, Y, Kato, K. Yamaguchi, Y. Hibino, K. Hirai and K. Kondo
Enantioselective synthesis of (S)-amino acids by phenylalanine dehydrogenase from Bacillus sphaericus: Use of natural and recombinant enzymes. J. Org. Chem., 55, 5567-5571 (1990).

Y. Asano
Enzymatic production of D-amino acids and their derivatives (in Japanese), Bioscience and Industry, 48, 131-137 (1990).

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2000, 1999, 1998, 1997, 1996, 1995, 1994, 1993, 1992, 1991
1990, 1989, 1988, 1987, 1986, 1985, 1984, 1982, 1981, 1980, 1979, 1977, 1975

1989

A. J. L. Cooper, L. K. H. Leung and Y. Asano
Enzymatic cycling assay for phenylpyruvate. Anal. Biochem., 183, 210-214 (1989).

Y. Asano, A. Nakazawa, Y. Kato and K. Kondo. Isolierung einer D-stereospezifischen Aminopeptidase und ihre Anwendung als Katalysator in der Organischen Synthese. Angew. Chem., 101, 511-512 (1989).

Y. Asano, T. Mori, S. Hanamoto, Y. Kato and A. Nakazawa
A New D-sereospecific amino acid amidase from Ochrobactrum anthropi. Biochem. Biophys. Res. Commun., 162, 470-474 (1989).

Y. Asano, K. Yamaguchi and K. Kondo
A New NAD⁺-dependent opine dehydrogenase from Arthrobacter sp. strain 1C. J. Bacteriol., 171, 4466-4471 (1989).

Y. Asano, A. Nakazawa, Y. Kato and K. Kondo
Properties of a novel D-stereospecific aminopeptidase from Ochrobactrum anthropi. J. Biol. Chem.,264, 14233-14239 (1989).

Y. Kato, Y. Asano, A. Nakazawa and K. Kondo
First stereoselective synthesis of D-amino acid amide catalyzed by a novel aminopeptidase. Tetrahedron, 45 (18), 5743-5754 (1989).

1988

N. Okazaki, Y. Hibino, Y. Asano, M. Ohmori, N. Numao and K. Kondo
Cloning and nucleotide sequencing of phenylalanine dehydrogenase gene of Bacillus sphaericus. Gene, 63 (2), 337-341 (1988).

Y. Asano, T. Sekigawa, M. Inukai and A. Nakazawa
Purification and properties of formate fehydrogenase from Moraxella sp. strain C-1. J. Bacteriol., 170, 3189-3193 (1988). >

Y. Asano and K. Endo
Amino acid racemase with broad substrate specificity -its properties and use in Phenylalanine Racemization-. Appl. Microbiol. Biotechnol., 29, 523-527 (1988).

1987

Y. Asano, A. Nakazawa and K. Endo
Novel phenylalanine dehydrogenases from Sporosarcina ureae and Bacillus sphaericus -purification and characterization-. J. Biol. Chem., 262, 10346-10354 (1987).

Y. Asano and A. Nakazawa
High yield synthesis of L-amino acids by phenylalanine dehydrogenase from Sporosarcina ureae. Agric. Biol. Chem., 51, 2035-2036 (1987).

Y. Asano, A. Nakazawa, K. Endo, Y. Hibino, M. Ohmori, N. Numao and K. Kondo
Phenylalanine dehydrogenase from Bacillus badius -purification, characterization and gene cloning-. Eur. J. Biochem., 168 (1), 153-159 (1987).

Y. Asano, K. Endo, A. Nakazawa, Y. Hibino, M. Ohmori, N. Numao and K. Kondo
Bacillus phenylalanine dehydrogenase produced in Escherichia coli -Its purification and application to L-phenylalanine synthesis-. Agric. Biol. Chem., 51, 2621-2623 (1987).

1986

A. K. Bandyopadhyay, T. Nagasawa, Y. Asano, K. Fujishiro, Y. Tani and H. Yamada
Purification and characterization of benzonitrilases from Arthrobacter sp. strain J-1. Appl. Environ. Microbiol., 51, 302-306 (1986).

1985

Y. Asano and A. Nakazawa
Crystallization of phenylalanine dehydrogenase from Sporosarcina ureae. Agric. Biol. Chem., 49, 3631-3632 (1985).

Y. Asano, J. J. Lee, T.-L. Shieh, F. Spreafico, C. Kowal and H. G. Floss
Steric course of the reactions catalyzed by 5-enolpyruvylshikimate-3-phosphate synthase, chorismate mutase, and Anthranilate Synthase. J. Am. Chem. Soc., 107, 4314-4320 (1985).

1984

Y. Asano, R. W. Woodard, D. R. Houck and H. G. Floss
Stereochemical course of the transmethylation catalyzed by histamine N-methyltransferase. Arch. Biochem. Biophys., 231 (1), 253-256 (1984).

J. J. Lee, Y. Asano, T.-L. Shieh, F. Spreafico and H. G. Floss
Steric course of the 5-enolpyruvylshikimate-3-phosphate synthetase and anthranilate synthetase reactions. J. Am. Chem. Soc., 106, 3367-3368 (1984).

1982

Y. Asano, K. Fujishiro, Y. Tani and H. Yamada
Aliphatic nitrile hydratase from Arthrobacter sp. J-1 -purification and characterization-. Agric. Biol. Chem., 46, 1165-1174 (1982).

Y. Asano, M. Tachibana, Y. Tani and H. Yamada
Purification and characterization of amidase which participates in nitrile degradation. Agric. Biol. Chem., 46, 1175-1182 (1982).

Y. Asano, T. Yasuda, Y. Tani and H. Yamada
A new enzymatic method of acrylamide production. Agric. Biol. Chem., 46, 1183-1189 (1982).

1981

Y. Asano, S. Ando, Y. Tani and H. Yamada
Fungal degradation of triacrylonitrile. Agric. Biol. Chem., 45, 57-62 (1981).

1980

H. Yamada, Y. Asano and Y. Tani
Microbial utilization of glutaronitrile. J. Ferment. Technol., 58, 495-500 (1980).

Y. Asano, Y. Tani and H. Yamada
A new enzyme "nitrile hydratase" which degrades acetonitrile in combination with amidase. Agric. Biol. Chem., 44, 2251-2252 (1980).

Y. Asano, S. Ando, Y. Tani and H. Yamada
Degradation of dinitriles by Fusarium merismoides TG-1. Agric. Biol. Chem., 44, 2497-2498 (1980).

1979

H. Yamada, Y. Asano, T. Hino and Y. Tani
Microbial utilization of acrylonitrile. J. Ferment. Technol., 57, 8-14 (1979).

1977

Y. Izumi, Y. Asano, Y. Tani and K. Ogata
Formation of valine and leucine by analog-resistant mutants of an obligate methylotroph, Methylomonas aminofaciens. J. Ferment. Technol. 55, 452-458 (1977).

K. Ogata, Y. Izumi, M. Kawamori, Y. Asano and Y. Tani
Amino acid formation by methanol-utilizing bacteria. J. Ferment. Technol., 55, 444-451 (1977).

1975

K. Nishimura, Y. Asano and M. Nakajima
Phagocidal activity of the hydrolyzate of acetal derivatives of oxidized spermine and iIts N,N'-dimethyl analog. Agric. Biol. Chem., 39, 1663-1666 (1975).

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2010, 2009, 2008, 2007, 2006, 2005, 2004, 2003, 2002, 2001

2000, 1999, 1998, 1997, 1996, 1995, 1994, 1993, 1992, 1991
1990, 1989, 1988, 1987, 1986, 1985, 1984, 1982, 1981, 1980, 1979, 1977, 1975

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Professor Yasuhisa Asano
Biotechnology Research Center
Toyama Prefectural University
5180 Kurokawa, Imizu,
Toyama 939-0398 JAPAN
Tel: +81-766-56-7500 ext 530; Fax: +81-766-56-2498
Mail to: asano@ pu-toyama.ac.jp