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RECENT PUBLICATIONS

2006, 2005, 2004, 2003, 2002, 2001, 2000, 1999, 1998,
1997, 1996, 1995, 1994, 1993, 1992, 1991, 1990

2006

S. Nanda, Y. Kato, and Y. Asano
PmHNL catalyzed synthesis of (R)-cyanohydrins derived from aliphatic aldehydes, Tetrahedron: Asymmetry, in press.

Y. Asano
Enzymes acting on D-amino acid amides, N. Fujii, H. Homma, H. Bruecker, G. H. Fisher, A. D'Aniello, and R. Konno (eds.). In D-Amino acids: A new frontier in amino acid and protein research, Nova Science Publishers, submitted.

2005

J. Sharma, D. Batovska, Y. Kuwamori, and Y. Asano
Enzymatic chemo-selective synthesis of secondary amide surfactant from N-methylethanol amine, J. Biosci. Bioeng., 100, (2005), in press.

Y. Asano, K. Tamura, N. Doi, T. Ueatrongchit, A. H-Kittikun, and T. Ohmiya
Screening for new hydroxynitrilases from plants, Biosci. Biotech. Biochem.,, 69 (12), 2349-2357 (2005).

Y. Kato, and Y. Asano
Purification and characterization of aldoxime dehydratase of the head blight fungus, Fusarium graminearum, Biosci. Biotech. Biochem.,, 69 (11), 2254-2257 (2005).

S. Nanda, Y. Kato, and Y. Asano
A new (R)-hydroxynitrile lyase from Prunus mume: asymmetric synthesis of cyanohydrins, Tetrahedron, 61 (46), 10908-10916 (2005).

Y. Asano and S. Yamaguchi
Discovery of amino acid amides as new substrates for a-amino-e-caprolactam racemase from Achromobacter obae, J. Molec. Catal. B: Enzymatic.,, 36 (1-6), 22-29 (2005).

R. Villalonga, S. Tachibana, Y. Perez, and Y. Asano
Increased conformational and thermally stable properties for phenylalanine dehydrogenase by chemical glycosidation with end-group activated dextran, Biotechnol. Lett., 27 (17), 1311-1317 (2005).

Y. Kato and Y. Asano
Molecular and enzymatic analysis of the "aldoxime-nitrile pathway" in the glutaronitrile degrader Pseudomonas sp. K-9, Appl. Microbiol. Biotechnol., published on line.

H. Komeda, N. Hariyama and Y. Asano
L-Stereoselective amino-acid amidase with broad substrate specificity from Brevundimonas diminuta: A new member of the leucine aminopeptidase family, Appl. Microbiol. Biotechnol., published on line.

H. Komeda and Y. Asano
A DmpA-homologous protein from Pseudomonas sp. is a dipeptidase specific for a-alanyl dipeptides. FEBS J., 272, 3075-3084 (2005).

A. Inoue, H. Komeda, and Y. Asano
Asymmetric synthesis of L-a-methylcysteine with the amidase from Xanthobacter flavus NR303, Advanced Synthesis & Catalysis, 347, 1132-1138 (2005).

T. Hongpattarakere, H. Komeda, and Y. Asano
Purification, characterization, gene cloning and nucleotide sequencing of D-stereospecific amino acid amidase from soil bacterium: Delftia acidovorans, J. Industrial Microbiol. Biotechnol.,, 32 (6), 567-576 (2005).

Y. Asano and S. Yamaguchi
Dynamic kinetic resolution of amino acid amide catalyzed by D-aminopeptidase and a-amino-e-caprolactam racemase, J. Am. Chem. Soc., 127 (21), 7696-7697 (2005).

Y. Kato, S. Yoshida, and Y. Asano
Polymerase chain reaction for identification of aldoxime dehydratase in aldoxime- or nitrile-degrading microorganisms, FEMS Microbiol. Lett., 246 (2), 243-249 (2005).

Y. Asano, I. Kira, and K. Yokozeki
Alteration of substrate specificity of aspartase by directed evolution, Biomolecular Engineering, 22 (1-3), 95-101 (2005).

K. Kobayashi, S. Yoshioka, Y. Kato, Y. Asano, and S. Aono
Regulation of Aldoxime Dehydratase Activity by Redox-dependent Change in the Coordination Structure of the Aldoxime-Heme Complex, J. Biol. Chem., 280 (7), 5486-5490 (2005).

Y. Asano
Enzymatic synthesis of (S)-phenylalanine and related (S)-amino acids by phenylalanine dehydrogenase, Microbial Enzymes and Biotransformations, J. L. Barredo, (ed.). In Methods in Biotechnology, Humana Press (2005), pp 157-166.

Y. Asano
Development of D-stereoselective amino acid amido hydrolases and their application to the dynamic kinetic resolution of amino acid amides. Pharmacia, (in Japanese), 41 (9), 881-884 (2005).

Y. Asano
Establishment of the Department of Biotechnology, Toyama Prefectural University and "Green Biotechnology" (in Japanese), In Toyama Monthly Economy, 8, (2005), published online.

S. Okazaki, T. Hikage, A. Suzuki, H. Komeda, Y. Asano, and T. Yamane
Structure determination of D-stereospecific amino acid amidase (in Japanese), In Progress in X-ray diffraction reseach, Nagoya University, Japan (2005), pp 21-23

H. Komeda, N. Mizuguchi, I. Kira, K. Yokozeki, and Y. Asano
Cloning of isoasparaginase ( a-aspartyl dipeptidase) gene from Escherichia coli K-12 and overexpression of the gene in E. coli. Bull. Toyama Pref. Univ., 15, 91-98 (2005).

Y. Asano
Enzyme Engineering, Chapters 1 and 4 (in Japanese) (Tokyo Kagakudojin Press), in press.

Y. Asano
Enzyme handbook, (EC 4.1.2.10, 4.1.2.11, 4.2.1.31, 4.2.1.32, 4.2.1.33, 4.2.1.34, 4.2.1.35, 4.2.1.65, 4.2.1.71, 4.2.1.72, 5.3.3.6) (in Japanese)(Asakura Press), in press.

Y. Asano and Y. Kato
Enzyme handbook, (EC 4.3.1.2) (in Japanese)(Asakura Press), in press.

Y. Asano and H. Komeda
Enzyme handbook, (EC 4.2.1.66, 4.2.1.69, 4.2.1.70, 4.2.1.73, 4.2.1.85) (in Japanese)(Asakura Press), in press.

Y. Asano, H. Ohta, and K. Ishihara
Selected abstracts from the 8th Japanese symposium on the chemistry of biocatalysis. J. Molec. Catal. B: Enzymatic., 36 (1-6), 54-74 (2005).

2004

Y. Mihara, K. Ishikawa, E. Suzuki, and Y. Asano
Improving the pyrophosphate-inosine phosphotransferase activity of Escherichia blattae acid phosphatase by sequential site-directed mutagenesis, Biosci. Biotechnol. Biochem., 68 (5), 1046-1050 (2004).
[pdf]

D. I. Batovska, S. Tsubota, Y. Kato, Y. Asano, and M. Ubukata
Lipase-mediated desymmetrization of glycerol with aromatic and aliphatic anhydrides, Tetraherdon Asymmetry, 150 (22), 3551-3559 (2004).
[pdf]

Y. Asano, Y. Kato, C. Levy, P. Baker, and D. Rice
Structure and function of amino acid ammonia-lyases, Biocatal. Biotransform., 22 (2), 133-140 (2004).

H. Komeda, H. Harada, S. Washika, T. Sakamoto, M. Ueda, and Y. Asano
A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide, Eur. J. Biochem., 271 (8), 1580-1590 (2004).
[pdf]

H. Komeda, H. Harada, S. Washika, T. Sakamoto, M. Ueda, and Y. Asano
S-Stereoselective piperazine-2-tert-butylcarboxamide hydrolase from Pseudomonas azotoformans IAM 1603 is a novel L-amino-acid amidase, Eur. J. Biochem., 271 (8), 1465-1475 (2004).
[pdf]

Y. Kato, S. Yoshida, S.-X. Xie, and Y. Asano
Aldoxime dehydratase co-existing with nitrile hydratase and amidase in iron-type nitrile hydratase producer Rhodococcus sp. N-771, J. Biosci. Bioeng., 97 (4), 250-259 (2004).
[pdf]

Y. Asano, N. Mizuguchi, H. Komeda, I. Kira, and K. Yokozeki
Identification of Isoasparaginase from Escherichia coli K-12 as a-aspartyl depetidase, Bull. Toyama Pref. Univ., 14, 96-103 (2004).

H. Komeda and Y. Asano
Functions of the D-stereoselective peptidases and their applications (in Japanese), Bioscience and Industry., 62, 591-594 (2004).

H. Komeda and Y. Asano
Screening for the stereoselective amino acid amidsaes and their use for the synthesis of optically active amino acids (in Japanese), Seibutsu Kougaku Kaishi, 82, 237-240 (2004).

Y. Asano and H. Kaga
Selected Abstracts from the 7th Japanese Symposium on the Chemistry of Biocatalysis. J. Molec. Catal. B: Enzymatic, 30(5-6), 241-256 (2004).
[pdf]

2003

S. Y. Seah, K. L. Britton, D. W. Rice, Y. Asano, P. C. Engel
Kinetic analysis of phenylalanine dehydrogenase mutants designed for aliphatic amino acid dehydrogenase activity with guidance from homology-based modelling, Eur. J. Biochem., 270 (23), 4628-4634 (2003).
[pdf]

K. Wada and Y. Asano
Use of borate to control the 5'-position selective microbial glucosylation of pyridoxine, Appl. Environ. Microbiol., 69 (12), 7058-7062 (2003).
[pdf]

H. Komeda and Y. Asano
Genes for an alkaline D-stereospecific endopeptidase and its homologue are located in tandem on Bacillus cereus genome, FEMS Microbiol. Lett., 228 (1), 1-9 (2003).
[pdf]

S.-X. Xie, Y. Kato, H. Komeda, S. Yoshida, and Y. Asano
A novel gene cluster responsible for alkylaldoxime metabolism coexisting with nitrile hydratase and amidase in Rhodococcus globerulus A-4, Biochemistry, 42(41), 12056-12066 (2003).
[pdf]

Y. Kato and Y. Asano
The high-level expression of a novel FMN-dependent heme-containing lyase, phenylacetaldoxime dehydratase of Bacillussp. Strain OxB-1, in heterologous hosts, Protein Expr. Purif., 28 (1), 131-139 (2003).
[pdf]

D.-H. Baek, J.-J. Song, S.-G. Lee, S.-J. Kwon, Y. Asano, and M.-H. Sung
New thermostable D-methionine amidase from Brevibacillus borstelensis BCS-1 and its application for D-phenylalanine production, Enzyme Microb. Technol., 32 (1), 131-139 (2003).
[pdf]

K. Wada and Y. Asano
Improvement of 5'-position selective glucosylation of pyridoxine by Verticillium dahliae TPU4900, Biosci. Biotech. Biochem., 67 (3), 508-516 (2003).
[pdf]

Y. Asano and K. Wada
Regioselective glucosylation of pyridoxine by microorganisms, Biosci. Biotech. Biochem., 67 (3), 499-507 (2003).
[pdf]

H. Komeda, N. Ishikawa, and Y. Asano
Enhancement of the thermostability and catalytic activity of D-stereospecific amino-acid amidase from Ochrobactrum anthropi SV3 by directed evolution, J. Molec. Catal. B: Enzymatic., 21 (4-6), 283-290 (2003).
[pdf]

Y. Asano
Two solutions in the structure and function of amino acid ammonia-lyases, Seibutsu-Kogaku Kaishi (in Japanese), 81 (5), 193 (2003).

Y. Asano
Chorismate mutase , In "Protein Chemistry" (in Japanese), T. Fukui and M. Itoh Eds., Hirokawa Press, vol 4, pp. 181-185 (2003), ISBN4-567-16047-9.

Y. Asano
Structure and function of amino acid ammonia-lyases, Kagaku to Kogyo (Osaka) (in Japanese), 77 (2), 71-78 (2003).

Y. Asano
Let soil microorganisms work!, Kagaku-Kogyo (Chemical Industry) (in Japanese), 54 (2), 124-127 (2003).

Y. Asano and K. Nakamura
Selected Abstracts from the 6th Japanese Symposium on the Chemistry of Biocatalysis. J. Molec. Catal. B: Enzymatic, 22 (3-4), 225-250 (2003).
[pdf]

2002

S.-Y. Seah, K. L. Britton, D. W. Rice, Y. Asano, and P. C. Engel
Single amino acid substitution in Bacillus sphaericus phenylalanine dehydrogenase dramatically increases its discrimination between phenylalanine and tyrosine substrates, Biochemistry, 41(38), 11390-11397 (2002).
[pdf]

C. W. Levy, P. A. Buckley, S. Sedelnikova, Y. Kato, Y. Asano, D. W. Rice, and P. J. Baker
Insights into enzyme evolution revealed by the structure of methylaspartate ammonia lyase, Structure, 10, 105-113 (2002).
[pdf].

K. Ishikawa, E. Suzuki, Y. Mihara, T. Utagawa, and Y. Asano
Enhancement of nucleoside phosphorylation activity in an acid phosphatase, Protein Engineering, 15(7), 539-543 (2002).
[pdf].

E. Omidinia, H. Taherkhani, Y. Asano, S. Khathami, A. Omumi, A.-A. Ghadiri, D. van der Lelie, R. Rashid Pouraie, H. Mirzahoseini, and A. Samadi
Affinity purification and characterization of recombinant Bacillus sphaericus phenylalanine dehydrogenase produced by using pET expression vector system. Iranian Biomedical Journal, 6 (1), 31-36 (2002).

E. Omidinia, A. Samadi, H. Taherkhani, S. Khathami, N. Moazami, R. Rashid Pouraie, and Y. Asano
Cloning and expression of Bacillus sphaericusphenylalanine dehydrogenase gene in Bacillus subtiliscells: purification and enzyme properties, World J. Microbiol. Biotechnol., 18(7), 593-597 (2002).

E. Omidinia, F. Mahboundi, S. Khathami, D. van der Lelie, N. Moazami, Y. Asano, and A. Samadi
Molecular cloning and evaluation of wild promoter in expression of Bacillus sphaericus phenylalanine Dehydrogenase gene in Bacillus subtilis cells. Journal of Sciences (Iranian Journal) (Tehran, Iran), 12 (4), 307-313 (2002).

H. Komeda and Y. Asano
Discovery of D-stereospecific peptidases of microbial origin: what are their roles? (in Japanese) Chemistry and Biology (Kagaku to Seibutsu, Gakkai Syuppan Center Press), 10(7), 429-431 (2002).

Y. Kato and Y. Asano
New insights for amino acid ammonia-lyases (in Japanese) Bioscience and Industry (in Japanese) 60(9), 32-33 (2002).

Y. Asano
Screening for microbial enzymes and thier fine tuning to application -Phosphorylation of nucloside by mutated acid phosphatase-, The Hokuriku Journal of Radioisotope Research (in Japanese), 4, 46-47 (2002).

Y. Asano and Y. Kato
Screening for industrial enzymes and fine tuning of thier activities, In "Green Biotechnology -Biotechnolgy for sustainable society" (in Japanese), H. Un'no and Y. Okahata Eds., Kodan-sya Press, pp. 71-82 (2002), ISBN4-06-154310-5.

Y. Asano
Overview of screening for new microbial catalysts and their uses in organic synthesis -Selection and optimization of biocatalysts-, J. Biotechnol., 94 (1), 65-72 (2002).
[pdf]

Y. Asano and Y. Ito
Selected Abstracts from the 5th Japanese Symposium on the Chemistry of Biocatalysis. J. Molec. Catal. B: Enzymatic., 18(1-3), 173-197 (2002).
[pdf]

2001

C. W. Levy, P. A. Buckley, P. J. Baker, S. Sedelnikova, F. Rogers, Y.-F. Li, Y. Kato, Y. Asano, and D. W. Rice
Crystallisation and preliminary X-ray analysis of Citrobacter amalonaticus methylaspartate ammonia Lyase, Acta Cryst. D., D57, 1922-1924 (2001).

S.-X. Xie, Y. Kato, and Y. Asano
High yield synthesis of nitriles by a new enzyme phenylacetaldoxime dehydratase from Bacillus sp. strain OxB-1, Biosci. Biotech. Biochem., 65 (12), 2666-2672 (2001).
[pdf]

Y. Mihara, T. Utagawa, H. Yamada, and Y. Asano
Acid phosphatase/phosphotransferases from enteric bacteria, J. Biosci. Bioeng., 92 (1), 50-54 (2001).
[pdf]

J.-H. Xu, Y. Kato and Y. Asano
Efficient preparation of (R)-a-Monobenzoyl glycerol by lipase catalyzed asymmetric esterification: optimization and operation in packed bed reactctor, Biotechnol. Bioeng., 73 (6), 493-499 (2001).
[pdf]

Y. Kato and Y. Asano
A new enzymatic method of stereoselective oxidation of racemic 1,2-indandiols, J. Molec. Catal. B: Enzymatic., 13 (1-3), 27-36 (2001).
[pdf]

Y. Asano and S. Watanabe
Isolation of Poly(3-Hydroxybutyrate) (PHB)-Depolymerase from Arthrobacter sp. Strain W6, Biosci. Biotech. Biochem.,65 (5), 1191-1194 (2001).
[pdf]

Y. Kato and Y. Asano
Occurrence of a novel lyase catalyzing b-elimination reaction toward threo-3-chloro-L-aspartate in Pseudomonas putida TPU 7151, Biosci. Biotech. Biochem.,65 (2), 435-437 (2001).
[pdf]

Y. Asano, M. Umezaki, Y.-F. Li, S. Tsubota, T. L. Luebbehuesen
Isolation of microorganisms utilizing acidic D-amino acid oligomers. J. Molec. Catal. B: Enzymatic, 12 (1-6), 53-59 (2001).
[pdf]

Y. Asano
Biotechnology 2000 (in Japanese), Enzyme Engineering News, 45, 45-46 (2001).

Y. Asano
Selected abstracts from the 3rd Japanese symposium on the chemistry of biocatalysis. J. Molec. Catal. B: Enzymatic., 14 (1-3), 45-66 (2001).

Preface to a special issue "D-Amino acid bioscience and biotechnology." J. Molec. Catal. B: Enzymatic, 12, 1 (2001). [pdf]

2006, 2005, 2004, 2003, 2002, 2001, 2000, 1999, 1998, 1997, 1996, 1995, 1994, 1993, 1992, 1991, 1990

2000

K. Ishikawa, Y. Mihara, K. Gondoh, E. Suzuki, and Y. Asano
X-ray structures of a novel acid phosphatase from Escherichia blattaeand its complex with the transition-state analog molybdate, EMBO J.,19 (11), 2412-2423 (2000).
[pdf]

Y. Mihara, T. Utagawa, H. Yamada, and Y. Asano
Phosphorylation of nucleosides by the mutated acid phosphatase from Morganella morganii, Appl. Environ. Microbiol., 66 (7), 2811-2816 (2000).
[pdf]

Y. Kato, R. Ooi, and Y. Asano
Distribution of aldoxime dehydratase in Microorganisms, Appl. Environ. Microbiol., 66, 2290-2296 (2000).
[pdf]

H. Komeda and Y. Asano
Gene cloning, Nucleotide Sequencing, and Purification and Characterization of the D-stereospecific amino acid amidase from Ochrobactrum anthropi SV3., Eur. J. Biochem., 267, 2028-2036 (2000).
[pdf]

Y. Kato, I. Fujiwara, and Y. Asano
Synthesis of optically active a-monobenzoyl glycerol by asymmetric transesterification of glycerol. J. Molec. Catal. B: Enzymatic, 9, 193-200 (2000).
[pdf]

Y. Kato, K. Nakamura, H. Sakiyama, S. G. Mayhew, and Y. Asano
A novel heme-containing lyase, phenylacetaldoxime dehydratase from Bacillus sp. strain OxB-1: purification, characterization, and molecular cloning of the gene. Biochemistry, 39, 800-809 (2000).
[pdf]

Y. Asano and T. Luebbehuesen
Enzymes acting on D-Amino acid containing Peptides. J. Bioscience and Bioengineering, 89, 295-306 (2000).
[pdf]

Y. Asano
Selection and directed evolution of new microbial biocatalysts and their application to organic synthesis, Agric. Chem. Biotechnol., 43 (4), 207-210 (2000).

Y. Asano
New enzymes acting on peptides containing D-amino acids: thier properties and application, J. Microbiol. Biotechnol., 10 (5), 573-579 (2000).

H. Komeda, H. Harada, Y. Asano
Screening for 4,6-bisphenylazoresorcinol (4,6-Bis) degrading microorganisms (in Japanese). Bull. Toyama Pref. Univ., 10, 96-102 (2000).

Y. Mihara and Y. Asano
Phosphorylation of nucleosides by mutated acid phosphatase, Bioscience and Industry (in Japanese), 58 (12), 865-866(2000).

Y. Asano
Enzymes in Biotechnology (in Japanese). J. Phys. Chem. Earth Sci. (Toyama), 42, 3-6 (2000).

Preface to a special issue "Professor Saburo Fukui and his scientific achievement." J. Molec. Catal. B: Enzymatic, 10, 1-3 (2000). [pdf]

1999

Y. Kato, I. Fujiwara, and Y. Asano
A novel method for preparation of optically active a-monobenzoyl glycerol via lipase-catalyzed asymmetric transesterification of glycerol., Bioorg. Med. Chem. Lett., 9, 3207-3210 (1999).
[pdf]

Y. Asano, Y. Mihara, and H. Yamada
A novel nucleoside phosphorylating enzyme from Morganella morganii. J. Biosci. Bioeng., 87, 732-738 (1999).
[pdf]

Y. Kato, T. Tsuda, and Y. Asano
Nitrile hydratase involved in aldoxime metabolism from Rhodococcus sp. strain YH3-3: purification and characterization. Eur. J. Biochem., 263, 662-670 (1999).
[pdf]

Y. Kato and Y. Asano
A New Enzymatic Method of Nitrile Synthesis by Rhodococcus sp. strain YH3-3. J. Molec. Catal. B: Enzymatic, 6, 249-256 (1999).
[pdf]

Y. Asano, Y. Mihara, and H. Yamada
A New Enzymatic Method of Selective Phosphorylation of Nucleosides. J. Molec. Catal. B: Enzymatic, 6, 271-277 (1999).
[pdf]

H. Komeda and Y. Asano
Synthesis of D-Phenylalanine Oligopeptides Catalyzed by Alkaline D-Peptidase from Bacillus cereus DF4-B. J. Molec. Catal. B: Enzymatic, 6, 379-386 (1999).
[pdf]

H. Kawashima, S. Mitamura, Y. Kato, and Y. Asano
Microbial Production of Optically Active Indene Derivatives (In Japanese), Aromatics (Tokyo), 51 (9,10), 319-325 (1999).

Y. Asano and H. Komeda
D-Aminopeptidase and Alkaline D-peptidase. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), p.111-121 (1999).

Y. Asano
Phenylalanine Dehydrogenase.Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), p.1955-1963 (1999).

Y. Kato and Y. Asano
Opine Dehydrogenase and Secondary Amine Dicarboxylic Acids. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), p.1851-1858 (1999).

M. Ueda, H. Yamada, and Y. Asano
D-Malate. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), p.1579-1585 (1999).

Y. Asano
Selected Abstracts from the 2nd Japanese Symposium on the Chemistry of Biocatalysis. J. Molec. Catal. B: Enzymatic., 7, 311-345 (1999).


Y. Asano
Microbial aldoxime metabolism and the enzymatic synthesis of nitriles. Bioscience and Industry (in Japanese) 57, 251-252 (1999).

Y. Asano
Development of new microbial enzymes and their applications, Jap. J. Syn. Org. Chem. (in Japanese), 57, 1064-1072 (1999).

1998

K.L. Britton, H.F. Rogers, Y. Asano, T. Dairi, Y. Kato, T.J. Stillman, and D.W. Rice
Crystallisation of Arthrobacter sp. strain 1C Opine Dehydrogenase and Its Complex with NAD+. Acta Cryst. D. 54, 124-126 (1998).

K.L. Britton, Y. Asano, and D.W.Rice
The Crystal Structure of the N-(1-D-Carboxyethyl)-L-norvaline dehydrogenase from Arthrobacter sp. strain 1C. Nature Structural Biology, 5, 593-601 (1998).
[pdf]

Y. Asano and M. Tanetani
Thermostable Phenylalanine Dehydrogenase from a Mesophilic Microbacterium sp. strain DM86-1, Arch. Microbiol., 169, 220-224 (1998).

Y. Asano and Y. Kato
Z-Phenylacetaldoxime Degradation by a Novel Aldoxime Dehydratase from Bacillus sp. strain OxB-1. FEMS Microbiol. Lett., 158, 185-190 (1998).
[pdf]

Y. Kato, R. Ooi, and Y. Asano
Isolation and Characterization of a Bacterium Possessing a Novel Aldoxime- Dehydration Activity and Nitrile-Degrading Enzymes. Arch. Microbiol., 170, 85-90 (1998)

Y. Kato, and Y. Asano
Cloning, Nucleotide Sequencing, and Expression of 3-Methylaspartate Ammonia Lyase Gene from Citrobacter amalonaticus strain YG-1002. Appl. Microbiol. Biotechnol., 50, 468-474 (1998)
[pdf]

Y. Asano
A Japanese Screening Approach -Selection of an Opine Dehydrogenase and Alkaline D-Peptidase-. Studies in Organic Chemistry 53, Proceedings of "New Frontiers in Screening for Microbial Biocatalysts" K. Kieslich, C.P. van der Beek, J.A.M. de Bont and W.J.J. van den Tweel, Ed., Elsevier (1998) 19-28.

Y. Asano
D-Aminopeptidase. Handbook of Proteolytic Enzymes (Academic Press), 430-433 (1998).

Y. Asano
Alkaline D-peptidase. Handbook of Proteolytic Enzymes (Academic Press), 433-435 (1998).

Y. Asano
Selected Abstracts from the 1st Japanese Symposium on the Chemistry of Biocatalysis. Journal of Molecular Catalysis B: Enzymatic., 5, 491-513 (1998).

Y. Asano and Y. Kato
Discovery of a new enzyme opine dehydrogenase and its application. Seibutsu Kogaku Kaishi, (in Japanese) 76, 426-429 (1998).

1997

Y. Kato and Y. Asano
3-Methylaspartate Ammonia-Lyase as a Maker Enzyme of the Mesaconate Pathway for (S)-Glutamate Fermentation in Enterobacteriaceae. Arch. Microbiol., 168, 457 463 (1997).

1996

Ivo Frebort, Hisanori Tamaki, Hiroki Ishida, Pavel Pec, Lenka Luhovク, Hiroko Tsuno, Martin Halata, Yasuhisa Asano, Yasuo Kato, Kazunobu Matsushita, Hirohide Toyama, Hidehiko Kumagai and Osao Adachi
Two Distinct Quinoprotein Amine Oxidases are Induced by n-Butylamine in the Mycelia of Aspergillus niger AKU 3302. Purification, Characterization, cDNA Cloning and Sequencing. Eur. J. Biochem., 237, 255-265 (1996).

Ivo Frebort, Pavel Pec, Lenka Luhovク, Hirohide Toyama, Kazunobu Matsushita, Shun Hirota, Teizo Kitagawa, Tamio Ueno, Yasuhisa Asano, Yasuo Kato, and Osao Adachi
Two Amine Oxidases from Aspergillus niger AKU 3302 Contain Topa Quinone as the Cofactor: Unusual Cofactor Link to the Glutamyl Residue Occurs Only at One of the Enzymes. Biochim. Biophys. Acta, 1295, 59-72 (1996).
[pdf]

K. Nakamura, T. Fujii, Y. Kato, Y. Asano and A. J. L. Cooper
Quantitation of L-Amino Acids by Substrate Recycling between an Aminotransferase and a Dehydrogenase: Application to the Determination of L-Phenylalanine in Human Blood. Anal. Biochem., 234, 19-22 (1996).
[pdf]

Y. Kato, H. Yamada and Y. Asano
Stereoselective Synthesis of Opine-type Secondary Amine Dicarboxylic Acids by a New Enzyme Opine Dehydrogenase - Use of Recombinant Enzymes-. J. Molec. Catal. B: Enzymatic, 1, 151-160 (1996).
[pdf]

Y. Kato, Y. Asano, and A. J. L. Cooper
Inactivation of Brain and Kidney Aspartate Aminotransferases by S-(1,2-Dichlorovinyl)-L-Cysteine and by S-(1,1,2,2-Tetrafluoroethyl)-L-Cysteine. Developmental Neuroscience, 18, 505-514 (1996).

Y. Kato, Y. Asano, Tapas K. Makar, and A. J. L. Cooper
Inhibition of Aspartate Aminotransferase by 2-Oxoglutaconic Acid and Its Dimethyl Ester. J. Biochem. (Tokyo), 120, 531-539 (1996).

Y. Asano, H. Ito, T. Dairi, and Y. Kato
An alkaline D-Stereospecific Endopeptidase with b-Lactamase Activity from Bacillus cereus. J. Biol. Chem., 271, 30256-30262 (1996).
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Y. Asano, M. Tanetani and N. Horita
Screening for Novel Amino Acid Dehydrogenases -Soil of Toyama Prefecture as a Source of Microorganisms- (in Japanese). Bull. Toyama Pref. Univ., 6, 101-106 (1996).

Y. Asano
Toyama Prefectural University (in Japanese), Chubu Bio Forum 10, 20-23 (1996).

Y. Asano
The second Japanese-Dutch workshop on biocatalysis (in Japanese), Enzyme Engineering News, 35, 34-35 (1996).

Y. Asano
Liquefaction of coal by enzymes and microorganisms (in Japanese), Chemistry and Industry, 49, 954 (1996).

1995

Y. Kato and Y. Asano
Purification and Properties of Crystalline 3-Methylaspartate Ammonia-lyase from Two Facultative Anaerobes, Citrobacter sp. Strain YG-0504 and Morganella morganii strain YG-0601. Biosci. Biotech. Biochem., 59, 93-99 (1995).

Y. Asano and K. Yamaguchi
Mutants of D-Aminopeptidase with Increased Thermal Stability. J. Ferment. Bioeng., 79, 614-616 (1995).

Y. Kato, Y. Asano and A. J. L. Cooper
3-Hydroxy-2-oxo-1-oxaspiro[4.5]-dec-3-ene, A New Inhibitor of Lactate Dehydrogenase. Tetrahedron Letters, 36, 4809-4812 (1995).
[pdf]

T. Dairi and Y. Asano
Cloning, Nucleotide Sequencing and Expression of Opine Dehydrogenase Gene from Arthrobacter sp. strain 1C. Appl. Environ. Microbiol., 61, 3196-3171 (1995).

S. Y.K. Seah, K. L. Britton, P. J. Baker, D. W. Rice, Y. Asano and P. C. Engel
Alternation in Substrate Specificity of Phenylalanine Dehydrogenase by Site Directed Mutagenesis. FEBS Letters, 370, 93-96 (1995).
[pdf]

A. Yamada, T. Dairi, Y.Ohno, X.-L. Huang and Y. Aasano
Nucleotide Sequencing of Phenylalanine Dehydrogenase Gene from Bacillus badius IAM 11059. Biosci. Biotech. Biochem., 59, 1994-1995 (1995).

Y. Kato and Y. Asano
3-Methylaspartate ammonia-lyase from a Facultative Anaerobe, strain YG-1002. Appl. Microbiol. Biotechnol., 43, 901-907 (1995).

H. Inoue, A. Takano, Y. Asano and Y. Katoh
Purification and Characterization of a 22-kDa Protein in Chloroplasts from Green Spores of the Fern Osmunda japonica. Physiol. Plant., 95, 465-471 (1995).

Y. Kato, J. Yamagishi and Y. Asano
Maleate cis-trans Isomerase from Arthrobacter sp. TPU 5446. J. Ferment. Bioeng., 80, 610-612 (1995).

Y. Kato, S. Ooe and Y. Asano
Screening for Novel Ammonia-lyases (in Japanese). Bull. Toyama Pref. Univ., 5, 114-126 (1995).

1994

M. Ueda, H. Yamada and Y. Asano
The Substrate Specificity of Maleate Hydratase from Arthrobacter sp. strain MCI2612. Appl. Microbiol. Biotechnol., 41, 215-218 (1994).

Y. Asano and Y. Kato
Crystalline 3-Methylaspartase from a Facultative Anaerobe,Escherichia coli strain YG1002. FEMS Microbiol. Lett., 118, 255-258 (1994).

Y. Asano and Y. Kato
Occurrence of 3-Methylaspartate ammonia-lyase in Facultative Anaerobes and Their Application to Synthesis of 3-Substituted (S)-Aspartic Acids. Biosci. Biotech. Biochem., 58 (1), 223-224 (1994).

Xin-Li Huang and Y. Asano
Screening for Novel Phenylalanine Dehydrogenases (in Japanese). Bull. Toyama Pref. Univ., 4, 211-215 (1994).

M. Ohto, A. Yamamoto, A. Matsunaga, Y. Asano, Y, Kawata, and E. Mizukami
Structure of Copper Chlorophyll Analogues in the Chewing Gum Product (in Japanese), Jpn. J. Toxicol. Environ. Health, 40, 279-285 (1994).

Y. Asano, Y. Yamamoto, and H. Yamada
Catechol-2,3-Dioxygenase-catalyzed Synthesis of Picolinic Acids from Catechols. Biosci. Biotech. Biochem., 58, 2054-2056 (1994).

Y. Asano, T. Komeda and H. Yamada
Enzymes Involved in Theobromine Production from Caffeine by Pseudomonas putida No.352. Biosci. Biotech. Biochem., 58, 2303-2304 (1994).

M. A. Smal., A. Cheung, Y. Asano, Z. Dong., L. Escoffier, M. Costello and H. N. Tattersall
Activation and Cytotoxicity of 2-a-Aminoacyl Prodrugs of Methotrexate. Biochem. Pharmacol., 49, 567-574 (1994).

Y. Asano
Biotechnology and agricultural chemistry, Nippon Nogei Kagaku Kaishi, 68, 1416-1417 (1994).

Y. Asano
Encyclopedia of Toyama (in Japanese), biotechnology, vitamin, gene manipulation, fermentation, enzyme, tissue culture, cell fusion, Kitanihon Shinbun (1994).

Y. Asano
Utilization of the functions of organisms (in Japanese), Science and Technology in Toyama (Toyama Prefecture), 12-17 (1994).

Y. Asano
Letting microorganisms synthesize useful materials (in Japanese), Science and Technology in Toyama (Toyama Prefecture), 118-119 (1994).

1993

Y. Asano, M. Ueda and H. Yamada
Microbial Production of D-Malate from Maleate. Appl. Environ. Microbiol., 59, 1110-1113 (1993).

Y. Kato, K. Fukumoto and Y. Asano
Enzymatic Synthesis of L-b-chloroalanine Using Amino Acid Dehydrogenase. Appl. Microbiol. Biotechnol., 39, 301-304 (1993).

Y. Asano, T. Komeda and H. Yamada
Microbial Production of Theobromine from Caffeine. Biosci. Biotech. Biochem., 57, 1286-1289 (1993).

M. Ueda, Y. Asano and H. Yamada
Purification and Characterization of Maleate Hydratase from Arthrobacter sp. strain MCI2612. Biosci. Biotech. Biochem., 57 (9), 1545-1548 (1993).

M. Ueda, R. Sashida, H. Yamada and Y. Asano
Production of S-(+)-Citramalate from Itaconate by Resting Cells of Alcaligenes denitrificans strain MCI2775. Appl. Microbiol. Biotechnol., 40, 466-469 (1993).

Y. Asano
The first Japanese-Dutch workshop on biocatalysis (in Japanese) , Bioscience and Bioindustry, 51, 911-912 (1993).

1992

Y. Asano, Y. Kato, A. Yamada and K. Kondo
Structural Similarity of D-Aminopeptidase to Carboxypeptidase DD and b-Lactamase. Biochemistry, 31, 2316-2328 (1992) .

Y. Asano
A New Enzyme D-Aminopeptidase: Structure, Function and Application to Organic Synthesis. Frontiers and New Horizons in Amino Acid Research, K. Takai Ed. (1992) 333-336, Elsevier

Y. Asano
Syntheses of amino acids by new microbial enzymes (in Japanese), Nippon Nogei Kagaku Kaishi, 66, 1117-1118 (1992).

Y. Asano, M. Ono, T. Sato, H. Nagaoka, J. Matsuda, S. Ogawa and T. Takeda
Current status and future perspective of synthetic organic chemistry (in Japanese), Japanese Journal of Synthetic Organic Chemistry, 50, 1116-1124 (1992) .

1991

Y. Asano, K. Kishino, A. Yamada, S. Hanamoto and K. Kondo
Plasmid-based, D-Aminopeptidase-catalyzed Synthesis of (R)-Amino Acids. Recl. Trav. Chim. Pays-Bas, 110, 206-208 (1991).

B. R. Blanco-De Guzman, Y. Asano and H. Yamada
Microbial Degradation of Opines: Isolation and Characterization of an Opine Utilizing Bacterium. Annual Reports of International Center of Cooperative Research and Development in Microbial Engineering, Japan, 14, 167-178 (1991).

Y. Asano
A new enzyme D-aminopeptidase -Structure, Its function and application to organic synthesis (in Japanese), Japanese Journal of Synthetic Organic Chemistry, 49, 314-326 (1991).

Y. Asano
Strecker amino acid synthesis (in Japanese), Japanese Journal of Synthetic Organic Chemistry, 49, 350 (1991).

Y. Asano
Enrich culture and acclimation techniques (in Japanese), Japanese Journal of Synthetic Organic Chemistry, 49, 350 (1991).

Y. Asano
Discovery of a new enzyme D-aminopeptidase and its application (in Japanese), Bioindustry, 8, 338-345 (1991).

Y. Asano
Discovery of a new enzyme D-aminopeptidase and its application to organic synthesis (in Japanese), Enzyme Engineering News, 25, 11-14 (1991).

Y. Asano
Syntheses of useful amides and amino acids by the use of new microbial enzymes (in Japanese), Nippon Nogei Kagaku Kaishi, 65, 710-712 (1991).

Y. Asano
Syntheses of useful amides and amino acids by the use of new microbial enzymes (in Japanese), Nippon Nogei Kagaku Kaishi, 65, 1617-1626 (1991).

1990

A. S. Gelbard, A. J. L. Cooper. Y. Asano, E. Nieves, S. Filc-DeRico and K. C. Rosenspire
Methods for the Enzymatic Synthesis of Tyrosine and Phenylalanine Labeled with Nitrogen-13. Appl. Rad. Isotopes, 41, 229-233 (1990).

Y. Kato, Y. Asano, A. Nakazawa and K. Kondo
Synthesis of D-alanine Oligopeptides by D-Aminopeptidase in Non-Aqueous Media. Biocatalysis, 3, 207-215 (1990).

Y. Asano, A. Yamada, Y, Kato, K. Yamaguchi, Y. Hibino, K. Hirai and K. Kondo
Enantioselective Synthesis of (S)-Amino Acids by Phenylalanine Dehydrogenase from Bacillus sphaericus: Use of Natural and Recombinant Enzymes. J. Org. Chem., 55, 5567-5571 (1990).

Y. Asano
Enzymatic production of D-amino acids and their derivatives (in Japanese), Bioscience and Industry, 48, 131-137 (1990).




2006, 2005, 2004, 2003, 2002, 2001, 2000, 1999, 1998, 1997, 1996, 1995, 1994, 1993, 1992, 1991, 1990


Professor Yasuhisa Asano
Biotechnology Research Center
Toyama Prefectural University
5180 Kurokawa, Kosugi,
Toyama 939-0398 JAPAN
Tel: +81-766-56-7500 ext 530; Fax: +81-766-56-2498
Mail to: asano@pu-toyama.ac.jp