RECENT PUBLICATIONS 2018, 2017, 2016, 2015, 2014, 2013, 2012, 2011
2010, 2009, 2008, 2007, 2006, 2005, 2004, 2003, 2002, 2001
2000, 1999, 1998, 1997, 1996, 1995, 1994, 1993, 1992, 1991
1990, 1989, 1988, 1987, 1986, 1985, 1984, 1982, 1981, 1980, 1979, 1977, 1975
P. Sakpetch, H. Komeda, Y. Asano, Y. Kuwahara, and Aran H-kittikun
Isolation of indigenous antagonistic microorganism to inhibit Rigidoporus microporus and other plant pathogens and analysis of the bioactive compounds. Biological Control, accepted.
Dohyun Im, D. Matsui, T. Arakawa, K. Isobe, Y. Asano, and S. Fushinobu
Ligand complex structures of L-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813 and its conformational change, FEBS Open Bio, accepted.
T. Yamaguchi, A. Nuylert, A. Ina, T Tanabe, and Y. Asano
Hydroxynitrile lyases from cyanogenic millipedes: molecular cloning, heterologous expression, and whole-cell biocatalysis for the production of (R)-mandelonitrile, Scientific Reports, accepted.
K. Isobe, S. Miki, R. Ueda, S. Shichida, D. Matsui, Y. Oku, and Y. Asano
Characterization of two carbonyl reductases from Ogataea polymorpha NBRC 0799 Appl. Microbiol. Biotechnol., First Online: 13 December 2017, doi.org/10.1007/s00253-017-8668-8
F. Motojima, A. Nuylert, and Y. Asano
The crystal structure and catalytic mechanism of hydroxynitrile lyase from passion fruit, Passiflora edulis, FEBS J., Published online, 7 December 2017. DOI: 10.1111/febs.14339
T. Motoyama, S. Nakano, Y. Yamamoto, H. Tokiwa, Y. Asano, and S Ito
Product release mechanism associated with structural changes in monomeric L-threonine 3-dehydrogenase, Biochemistry, Published online, 9 October 2017. DOI: 10.1021/acs.biochem.7b00832
T. Betke, P. Rommelmann, K. Oike, Y. Asano, and H. Groger
Cyanide-free and broadly applicable enantioselective synthetic platform for chiral nitriles through a biocatalytic approach, Angew. Chem. Int. Ed., 56 (40), 12361-12366 (2017). DOI: 10.1002/anie.201702952 [Back Cover], [Very Important Paper] *
D. Matsui, K. Fuhshuku, S. Nagamori, M. Takata, and Y. Asano
Isolation and characterization of racemase from Ensifer sp. 23-3 that acts on α-aminolactams and α-amino acid amides J. Ind. Microbiol. Biotechnol., 44 (11), 1503-1510 (2017). doi:10.1007/s10295-017-1981-5
D. Matsui, S. Nakano, M. Dadashipour, and Y. Asano
Rational identification of aggregation hotspots based on secondary structure and amino acid hydrophobicity, Scientific Reports, 7, 9558 (2017). DOI:10.1038/s41598-017-09749-2
K. Yamamoto, Y. Oku, A. Ina, A. Izumi, M. Doya, S. Ebata, and Y. Asano
Purification and characterization of a novel enone reductase from Sporidiobolus salmonicolor TPU 2001 reacting with large monocyclic enones, ChemCatChem, 9, 3697-3704 (2017). DOI: 10.1002/cctc.201700244
S. Yamasaki-Yashiki, H. Komeda, K. Hoshino, and Y. Asano
Characterization and gene cloning of L-xylulose reductase involved in L-arabinose catabolism from the pentose-fermenting fungus Rhizomucor pusillus, Biosci. Biotechnol. Biochem., 81 (8), 1612-1618 (2017). http://dx.doi.org/10.1080/09168451.2017.1320518
K. Yamamoto and Y. Asano
Genome sequence of a lumichrome producer, Microbacterium sp. strain TPU 3598, Genome announcement, 5 (16), e00204-17 (2017).
Y. Kuwahara, T. Tanabe, and Y. Asano
Changes in defense allomone compositions of Eutrichodesmus elegans and E. armatus (Polydesmida: Haplodesmidae) during different stages of their life cycles. Appl. Entomol. Zool., 52(3), 447-455 (2017). DOI: 10.1007/s13355-017-0478-z
Y. Kuwahara, T. Yamaguchi, Y. Ichiki, T. Tanabe, Y. Asano
Hydrogen peroxide as a new defensive compound in “benzoyl cyanide” producing polydesmid millipedes, Sci. Nat., 104 (19), (2017) doi:10.1007/s00114-017-1435-y
Y. Miki, S. Okazaki, and Y. Asano
Engineering an ATP-dependent D-Ala:D-Ala ligase for synthesizing amino acid amides from amino acids, J. Ind. Microbiol. Biotechnol., 44 (4), 667-675 (2017). DOI 10.1007/s10295-016-1833-8
W. Payoungkiattikun, S. Okazaki, A. Ina, A. H-Kittikun, and Y. Asano
Characterization of an α-amino-ε-caprolactam racemase with broad substrate specificity from Citreicella sp. SE45, J. Ind. Microbiol. Biotechnol., 44 (4), 677-685 (2017). DOI: 10.1007/s10295-016-1825-8.
Y-.f. Miao, R. Metzner, and Y. Asano
Kemp elimination catalyzed by naturally occurring aldoxime dehydratases, ChemBioChem, 18 (5), 451-454 (2017). DOI: 10.1002/cbic.201600596
A. Nuylert, Y. Ishida, and Y. Asano
Effect of glycosylation on the biocatalytic properties of hydroxynitrile lyase from the passion fruit, Passiflora edulis - a comparison of natural and recombinant enzymes, ChemBioChem, 18 (3), 257-265 (2017). DOI: 10.1002/cbic.201600447.
M. Kameya and Y. Asano
Translation-dependent bioassay for amino acid quantification using auxotrophic microbes as biocatalysts of protein synthesis, Appl. Microbiol. Biotechnol, 101 (6), 2523-2531 (2017). doi:10.1007/s00253-016-8027-1
T. Yamaguchi, Y. Kuwahara, and Y. Asano
A novel cytochrome P450, CYP3201B1, is involved in (R)-mandelonitrile biosynthesis in a cyanogenic millipede, FEBS Open Bio, 7 (3), 335-347 (2017). DOI: 10.1002/2211-5463.12170
W. Sukpipat, H. Komeda, P. Prasertsan, and Y. Asano
Purification and characterization of xylitol dehydrogenase with L-arabitol dehydrogenase activity from the newly isolated pentose-fermenting yeast Meyerozyma caribbica 5XY2, J. Biosci. Bioeng., 123 (1), 20-27 (2017).
N. Kawahara, K. Yasukawa, and Y. Asano
New enzymatic methods for the synthesis of primary α-aminonitriles and unnatural α-amino acids by oxidative cyanation of primary amine with D-amino acid oxidase from porcine kidney, Green Chem.,19, 418-424 (2017). DOI: 10.1039/C6GC02003H
Y. Asano, and R. Metzner
Exploiting natural diversity for industrial enzymatic applications, Royal Society of Chemistry, Catalysis Series, Ed. Gavin Williams and Mellanie Hall, in press (2017)
Y. Asano, and S. Okazaki
Enzymes in aldoxime-nitrile pathway: versatile tools in biocatalysis, In Future directions in bioctalysis, ver 2, Elsevier, New York, U.S.A., Ed. Tomoko Matsuda, Chapter 9, pp173-187 (2017).
Y. Asano and D. Matsui
Development of enzymes to determine amino acids by growth-dependent molecular selection (In Japanese), Fine Chemical, 46 (2), (2017).
S. Nakano, K. Yasukawa, T. Tokiwa, T. Ishikawa, E. Ishitsubo, N. Matsuo, S. Ito, H. Tokiwa, and Y. Asano
Origin of stereoselectivity and substrate/ligand recognition in an FAD-dependent R-selective amine oxidase, J. Phys. Chem. B, 120 (41), 10736-10743 (2016). DOI: 10.1021/acs.jpcb.6b09328.
Y. Ishida, Y. Kuwahara, M. Dadashipour, A. Ina, T. Yamaguchi, M. Morita, Y. Ichiki, and Y. Asano
A sacrificial millipede altruistically protects its swarm using a drone blood enzyme, mandelonitrile oxidase, Scientific Reports, 6, 26998 (2016). doi:10.1038/srep26998
T. Yamaguchi, and Y. Asano
Draft genome sequence of an aldoxime degrader, Rhodococcus sp. strain YH3-3, Genome announcements, 4(3), e00406-16 (2016). doi: 10.1128/genomeA.00406-16.
T. Yamaguchi, K. Noge, and Y. Asano
Cytochrome P450 CYP71AT96 catalyses the final step of herbivore-induced phenylacetonitrile biosynthesis in the giant knotweed, Fallopia sachalinensis, Plant Mol. Biol., 91 (3), 1-11 (2016). DOI: 10.1007/s11103-016-0459-6 Cover picture
D. Matsui, A. Terai, and Y. Asano
L-Arginine oxidase from Pseudomonas sp. TPU 7192: Characterization, gene cloning, heterologous expression, and application to L-arginine determination, Enzyme Microb. Technol., 82, 151-157 (2016). DOI: 10.1016/j.enzmictec.2015.10.002
Y. Asano, S. Okazaki, and K. Yasukawa
Recent developments in amino peptidases, oxidases and racemases, In Biocatalysis-Green Technology, Ed. Ramesh Patel, Chapter 19, pp489-502 (2016)
Y. Asano and N. Kawahara
A new S-hydroxynitrile lyase from Baliospermum montanum-Its structure, molecular dynamics simulation, and improvement by protein engineering, Industrial Biotechnology, 12 (2), 91-97 (2016). Cover picture
Y. Asano, S. Okazaki, and K. Yasukawa
Recent Developments in Amino peptidases, oxidases and racemases, In “Biocatalysis-Green Technology”, Ed. Ramesh Patel, John Wiley & Sons, Inc., Hoboken, New Jersey, 489-502 (2016).
Enchanted by Enzyme Discovery -Developing biological power-(In Japanese), Modern Chemistry (Gendai Kagaku), 539 (2), 48-53 (2016).
K. Yamamoto and Y. Asano
Efficient production of lumichrome by Microbacterium sp. Strain TPU 3598, Appl. Environ. Microbiol., 81 (21), 7360-7367 (2015). doi: 10.1128/AEM.02166-15
S. Nakano, S. Okazaki, E. Ishitsubo, N. Kawahara, H. Komeda, H. Tokiwa, and Y. Asano
Structural and computational analysis of peptide recognition mechanism of class-C type penicillin binding protein, alkaline D-peptidase from Bacillus cereus DF4-B, Scientific Reports, 5, 13836 (2015). doi:10.1038/srep13836
M. Dadashipour, Y. Ishida, K. Yamamoto, and Y. Asano
Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede Chamberlinius hualienensis, Proc. Natl. Acad. Sci. USA, 112 (34), 10605-10610 (2015). http://www.pnas.org/content/early/2015/08/05/1508311112.full.pdf "*Cover picture" doi: 10.1073/pnas.1508311112
N. Kawahara and Y. Asano
Mutagenesis of an Asn156 residue in a surface region of S-selective hydroxynitrile lyase from Baliospermum montanum enhances catalytic efficiency and enantioselectivity, ChemBioChem, 16 (13), 1891-1895 (2015). Published online: 27 JUL 2015 DOI: 10.1002/cbic.201500225, *Inside cover picture"
W. Payoungkiattikun, S. Okazaki, S. Nakano, A. Ina, A. H-Kittikun, and Y. Asano
In silico identification for α-amino-ε-caprolactam racemases by using Information on the structure and function relationship, Appl. Biochem. Biotechnol., 176 (5), 1303-1314 (2015). DOI 10.1007/s12010-015-1647-6
K. Fuhshuku, S. Watanabe, T. Nishii, A. Ishii, and Y. Asano
A novel S-enantioselective amidase acting on 3,3,3-trifluoro-2-hydroxy-2-methylpropanamide from Arthrobacter sp. S-2, Biosci. Biotechnol. Biochem., 79 (10), 1587-1596 (2015). DOI:10.1080/09168451.2015.1038216
D. Matsui, and Y. Asano
Heterologous production of L-lysine ε-oxidase by directed evolution using a fusion reporter method, Biosci. Biotechnol. Biochem., 79 (9), 11473-1480 (2015). DOI:10.1080/09168451.2015.1023251
S. Nakano, and Y. Asano
Protein evolution analysis of S-hydroxynitrile lyase by complete sequence design utilizing the INTMSAlign software, Scientific Reports, 5, 8193 (2015). doi:10.1038/srep08193.
T. Yamaguchi, and Y. Asano
Complete genome of an aldoxime degrader, Bacillus sp. OxB-1, Genome announcements, 3 (1) e00025-15 (2015). doi:10.1128/genomeA.00025-15.
D. Matsui, S. Okazaki, M. Matsda, and Y. Asano
Enhancement of stability of L-tryptophan dehydrogenase from Nostoc punctiforme ATCC29133 and its application to L-tryptophan assay, J. Biotechnol., 196-197, 27-32 (2015). doi:10.1016/j.jbiotec.2015.01.010
A. Sugawara, D. Matsui, M. Yamada, Y. Asano, and K. Isobe
Characterization of two amine oxidases from Aspergillus carbonarius AIU 205, J. Biosci. Bioeng., 119 (6), 629-635 (2015). doi:10.1016/j.jbiosc.2014.10.023
A. Sugawara, D. Matsui, M. Yamada, Y. Asano, and K. Isobe
New enzymatic methods for selective assay of L-lysine using an L-lysine specific decarboxylase/oxidase from Burkholderia sp. AIU 395, J. Biosci. Bioeng., 119 (3), 369-374 (2015). doi:10.1016/j.jbiosc.2014.08.013
H. Komeda, S. Yamasaki-Yashiki, K. Hoshino, and Y. Asano
Identification and characterization of D-xylose reductase involved in pentose catabolism of the zygomycetous fungus Rhizomucor pusillus, J. Biosci. Bioeng., 119 (1), 57-64 (2015). DOI: 10.1016/j.jbiosc.2014.06.012
Hydrolysis of Nitriles to Amides, In Science of Synthesis: Biocatalysis in Organic Synthesis, K. Faber, W.-D. Fessner, N. Turner, Eds, Georg Thieme Verlag KG., Vol. 1, pp 255-276 (2015).
A. Sugawara, D. Matsui, M. Yamada, Y. Asano, and K. Isobe
Characterization of a pyridoxal-5'-phosphate-dependent l-lysine decarboxylase/oxidase from Burkholderia sp. AIU 395 J. Biosci. Bioeng., 118 (5), 496-501 (2014). doi:10.1016/j.jbiosc.2014.04.013
S. Nakano, M. Dadashipour, and Y. Asano
Structural and functional analysis of hydroxynitrile lyase from Baliospermum montanum with crystal structure, molecular dynamics and enzyme kinetics, BBA Proteins and Proteomics, 1844 (12), 2059-2067 (2014). doi:10.1016/j.bbapap.2014.09.004
H. Komeda, S. Yamasaki-Yashiki, K. Hoshino, and Y. Asano
Identification and characterization of D-xylulokinase from the D-xylose-fermenting fungus, Mucor circinelloides, FEMS Microbiol. Lett., 360 (1), 51-61 (2014). DOI: 10.1111/1574-6968.12589
R. Metzner, S. Okazaki, Y. Asano, and H. Groger
Cyanide-free enantioselective eynthesis of nitriles: synthetic proof of a biocatalytic concept and mechanistic insights, ChemCatChem, 6 (11), 3105-3109 (2014). DOI: 10.1002/cctc.201402612
Y. Miki, and Y. Asano
Construction of the biosynthetic pathway for the cyanide-free production of phenylacetonitrile in Escherichia coli utilizing plant cytochrome P450 79A2 and bacterial aldoxime dehydratase, Appl. Environ. Microbiol., 80 (21), 6828-6836 (2014) doi: 10.1128/AEM.01623-14 (Spotlight: Articles of Significant Interest Selected from This Issue by the Editors).
T. Yamaguchi, K. Yamamoto, and Y. Asano
Identification and characterization of CYP79D16 and CYP71AN24 catalyzing the first and second steps in L-phenylalanine-derived cyanogenic glycoside biosynthesis in the Japanese apricot, Prunus mume Sieb. et Zucc, Plant Molec. Biol., 86 (1), 215-223 (2014). DOI:10.1007/s11103-014-0225-6
S. Yamasaki-Yashiki, H. Komeda, K. Hoshino, and Y. Asano
Molecular analysis of NAD+-dependent xylitol dehydrogenase from the zygomycetous fungus Rhizomucor pusillus and reversal of the coenzyme preference, Biosci. Biotechnol. Biochem., 78 (11),1943-1953 (2014). doi:10.1016/j.jbiosc.2014.06.012
Y. Kuwahara, Y. Ichiki, M. Morita, and Y. Asano
(2-Nitroethyl)benzene: a major flower scent from the Japanese Loquat Eriobotrya japonica [Rosales: Rosaceae], Biosci. Biotechnol. Biochem., 78 (8),1320-1323 (2014). DOI:10.1080/09168451.2014.921558
S. Nakano, S. Okazaki, H. Tokiwa, and Y. Asano
Binding of NAD+ and L-threonine induces stepwise structural and flexibility changes in Cupriavidus necator L-threonine dehydrogenase, J. Biol. Chem., 289 (15), 10445-10454 (2014). DOI: 10.1074/jbc.M113.540773
K. Yasukawa, S. Nakano, and Y. Asano
Tailoring D-amino acid oxidase from the pig kidney to R-stereoselective amine oxidase and its use in deracemization of a-methylbenzylamine, Angew. Chem. Int. Ed. Engl., 53 (17), 4428-4431 (2014). DOI: 0.1002/ange.201308812
A. Sugawara , D. Matsui, H. Komeda, Y. Asano, and K. Isobe
Characterization and application of aminoamide-oxidizing enzyme from Aspergillus carbonarius AIU 205, J. Biosci. Bioeng. 117 (3), 263-268 (2014). doi:10.1016/j.jbiosc.2013.08.019
D. Matsui, Do-Hyun Im, A. Sugawara, Y. Fukuta, S. Fushinobu, K. Isobe, and Y. Asano
Mutational and crystallographic analysis of L-amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813: Interconversion between oxidase and monooxygenase activities, FEBS Open Bio, 4, 220-228 (2014). DOI: 10.1016/j.fob.2014.02.002, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970082/
K. Fuhshuku, M. Takata, H. Iwatsubo, and Y. Asano
Preparation of D-a-aminolactams by L-enantioselective degradation of a-aminolactam mediated by Mesorhizobium sp. L88, Biocatal. Agric. Biotechnol., 3 (3), 42-47 (2014). doi:10.1016/j.bcab.2014.01.003
K. Fuhshuku, S. Watanabe, T. Nishii, A. Ishii, Y. Asano
Efficient preparation of both enantiomers of 3,3,3-trifluoro-2-hydroxy-2-methylpropanoic acid catalyzed by Shinella sp. R-6 and Arthrobacter sp. S-2, J. Molec. Catal. B: Enzymatic., 102, 115-119 (2014). doi:10.1016/j.molcatb.2014.01.024
M. Kameya and Y. Asano
Rapid enzymatic assays for L-citrulline and L-arginine based on the platform of pyrophosphate detection, Enzyme Microb. Technol., 57 (10), 36-41 (2014). doi:10.1016/j.enzmictec.2014.01.008
M. Kameya, M. Himi, and Y. Asano
Rapid and selective enzymatic assay for L-methionine based on a pyrophosphate detection system, Anal. Biochem., 447, 33-38 (2014). https://doi.org/10.1016/j.ab.2013.11.002
S. Kudo, Y. Kuwahara, Z. Korsos, Y. Ichiki, M. Morita, Y. Asano, Y. Nakamura, and T. Tanabe
Notes on egg brooding newly recorded in two millipede species (in Japanese), Jpn. J. Ent (N.S.), 17 (4), 135-138 (2014).
Y. Asano, K. Yasukawa, S. Nakano
Development of a new amine oxidase which had not been found in Nature (in Japanese), Chemistry (Kagaku), 69 (8), 45-49（2014).
Industrial application of new mirobial and plant enzymes responsible for degradation and biosynthesis of nitriles (in Japanese), Chemistry and Biology (Kagaku to Seibutsu), 52 (10), 651-658（2014).
Y. Asano, K. Yasukawa
Development of Enzymes for D-amino Acid Production, Bioindustry (in Japanese), 31(3), 49-56（2014).
S. Okazaki, S. Nakano, D. Matsui, S. Akaji, K. Inagaki and Y. Asano
X-Ray crystallographic evidence for the presence of the cysteine tryptophylquinone cofactor in L-lysine e-oxidase from Marinomonas mediterranea, J. Biochem., 154 (3), 233-236 (2013).
M. Kameya, H. Onaka, and Y. Asano
Selective tryptophan determination using tryptophan oxidases involved in bis-indole antibiotic biosynthesis, Anal. Biochem., 438 (2), 124-132 (2013).
8.4.1 Biocalatlysis in Engineering: Basic knowledge (in Japanese), In "Kagaku Binran" (Handbook of Chemistry) (Maruzen Co., Ltd.), (化学便覧 応用化学編 第7版』「8.4.1 バイオ触媒工学：基礎知識」丸善）, in press.
D-stereospecific aminopeptidase, In N. D. Rawlings and G. S. Salvesen (ed.), Handbook of Proteolytic Enzymes 3rd Ed. (Academic Press, London), Vol. 3, Chapter 767, pp 3463-3467 (2013).
Alkaline D-peptidase, In N. D. Rawlings and G. S. Salvesen (ed.), Handbook of Proteolytic Enzymes 3rd Ed. (Academic Press, London), Vol. 3, Chapter 768, pp 3467-3469 (2013).
K. Yasukawa and Y. Asano
Enzymatic Synthesis of Chiral Phenylalanine Derivatives by a Dynamic Kinetic Resolution of Corresponding Amide and Nitrile Substrates with a Multi-Enzyme System, Advanced Synthesis & Catalysis, 354 (17), 3327-3332 (2012).
H. Takeuchi, E. Ito, T. Tomioka, E. Tabuchi, K. Fuhshuku, and Y. Asano
Trans fatty acid intake and serum cholesterol levels in young Japanese women, Biosci. Biotech. Biochem., 76 (9), 1627-1632 (2012).
K. Isobe, A. Sugawara, H. Domon, Y. Fukuta, and Y. Asano
Purification and characterization of an L-amino acid oxidase from Pseudomonas sp. AIU 813, J. Biosci. Bioeng., 114 (3), 257-261 (2012).
S. Yamasaki-Yashiki, S. Tachibana, and Y. Asano
Determination of L-methionine using methionine-specific dehydrogenase for diagnosis of homocystinuria due to cystathionine b-synthase deficiency, Anal. Biochem., 428 (2), 143-149 (2012).
M. Matsuda and Y. Asano
A simple assay of taurine concentrations in food and biological sample using taurine dioxygenase, Anal. Biochem., 427 (2), 121-123 (2012).
K. Fuhshuku, and Y. Asano
Synthesis of optically active medium-sized a-aminolactams via ring-closing metathesis, Tetrahedron, 68 (33), 6651-6655 (2012).
Harald Groger, Yasuhisa Asano, Uwe T. Bornscheuer, and Jun Ogawa
Development of Biocatalytic Processes in Japan and Germany - From Research Synergies to Industrial Applications, Chemistry-An Asian Journal, 7, 1138-1153 (2012), DOI: 10.1002/asia.201200105.
Y. Asano and P. Kaul
Hydrolysis and reverse hydrolysis: Selective nitrile hydrolysis using nitrile hydratase and nitrilase, In Comprehensive Chirality, (Elsevier), In "Comprehensive Chirality", Ed. E. M. Carreira and H. Yamamoto (Elsevier), Volume 7, pp 122-142.
H. Groger and Y. Asano
1. Introduction - Principle and historial landmarks of enzyme catalysis in organic synthesis. In "Enzyme Catalysis in Organic Synthesis", Ed. K. Drauz, H. Groger and O. May (Springer Verlag), Volume 1, pp 3-42 (2012).
Y. Asano and K. Hoelsch
39. Isomerizations. In "Enzyme Catalysis in Organic Synthesis", Ed. K. Drauz, H. Groger and O. May (Springer Verlag), Volume 3, pp 1607-1684 (2012).
K. Fuhshuku, and Y. Asano
Organic synthesis catalyzed by plant enzyme hydroxynitrile lyase (in Japanese), Japanese Journal of Synthetic Organic Chemistry, 50, 102-112 (2012).
Enzymes acting on D-amino acid containing peptides. In L. Pollegioni and S. Servi (ed.) "Unnatural amino acids", "Methods in Molecular Biology" series 794 (Humana Press), pp 397-406 (2012).
Eftychia Pinakoulaki, Constantinos Koutsoupakis, Hitomi Sawai, Andrea Pavlou, Yasuo Kato, Yasuhisa Asano, and Shigetoshi Aono
Aldoxime dehydratase: probing the heme environment involved in the synthesis of the carbonnitrogen triple bond, J. Phys. Chem. B, 115, 13012-13018 (2011).
K. Yasukawa, R. Hasemi, and Y. Asano
Dynamic kinetic resolution of α-aminonitrile to form chiral α-amino acid, Advanced Synthesis & Catalysis, 353 (13), 2328-2332 (2011).
Yasuhisa Asano, Mohammad Dadashipour, Mizue Yamazaki, Nobutaka Doi, and Hidenobu Komeda
Functional expression of a plant hydroxynitrile lyase in Escherichia coli by directed evolution: Creation and characterization of highly in vivo soluble mutants, Protein Engineering Design and Selection, 24 (8), 607-616 (2011).
Ken-ichi Fuhshuku, and Yasuhisa Asano
Synthesis of (R)-β-nitro alcohols catalyzed by R-selective hydroxynitrile lyase from Arabidopsis thaliana in the aqueous-organic biphasic system, J. Biotechnol., 153 (3-4), 153-159 (2011).
Mohammad Dadashipour, Mizue Yamazaki, Kazumi Momonoi, Ken'ichirou Tamura, Ken-ichi Fuhshuku, Yurina Kanase, Etsuzoh Uchimura, Guan Kaiyun, Yasuhisa Asano
S-selective hydroxynitrile lyase from a plant Baliospermum montanum: Molecular characterization of recombinant enzyme, J. Biotechnol., 153 (3-4), 100-110 (2011).
Y. Fukuta, S. Nanda, Y. Kato, H. Yurimoto, Y. Sakai, H. Komeda, and Y. Asano
Characterization of a new (R)-hydroxynitrile lyase from the Japanese apricot Prunus mume and cDNA cloning and secretory expression of one of the isozymes in Pichia pastoris, Biosci. Biotech. Biochem., 75 (2), 214-220 (2011).
Mohammad Dadashipour, Yasuhisa Fukuta, and Yasuhisa Asano
Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems, Protein Expr. Purif., 77 (1), 92-97 (2011).
Techawaree Ueatrongchit, and Yasuhisa Asano
Highly selective L-threonine 3-dehydrogenase from Cupriavidus necator and its use in determination of L-threonine, Anal. Biochem., 410 (1), 44-56 (2011).
Mohammad Dadashipour and Yasuhisa Asano
Hydroxynitrile lyases: insights into biochemistry, discovery and engineering, ACS Catalysis, 1 (9), 1121-1149 (2011).
Praveen Kaul and Yasuhisa Asano
Strategies for discovery of novel enzymes: state of the art and opportunities, Microbial Biotechnology, 24 AUG 2011, DOI: 10.1111/j.1751-7915.2011.00280.x
Kimiyasu Isobe, Hiroshi Tamauchi, Ken-ichi Fuhshuku, Shouko Nagasawa and Yasuhisa Asano
A Simple enzymatic method for production of a wide variety of D-amino acids using L-amino acid oxidase from Rhodococcus sp. AIU Z-35-1, Enzyme Research, Article ID 567210 (2010) (online publication).
Motoki Matsuda, and Yasuhisa Asano
Determination of plasma and serum L-lysine using L-lysine e-oxidase from Marinomonas mediterranea NBRC103028T, Analytical Biochemistry, 406 (1), 19-23 (2010).
I.G. Khaliullin, D.A. Suplatov, D.N. Shalaeva, M. Otsuka, Y. Asano, and V.К. Svedas
Bioinformatic analysis and molecular modeling reveal a role of Lys65 residue in the catalytic triad of D-aminopeptidase from Ochrobactrum anthropi , Acta Naturae (Moscow), 2 (5), 62-66 (2010).
Techawaree Ueatrongchit, Ken'ichirou Tamura, Tohru Ohmiya, Aran H-Kittikun, and Yasuhisa Asano
Hydroxynitrile lyase from Passiflora edulis:Purification, characteristics and application in asymmetric synthesis of (R)-mandelonitrile, Enzyme and Microbial Technology, 46 (6), 456-465 (2010).
Yasuhisa Fukuta, Shinpei Koizumi, Hidenobu Komeda, and Yasuhisa Asano
A new aryl acylamidase from Rhodococcus sp. strain Oct1 acting on w-lactams: its characterization and gene expression in Escherichia coli, Enzyme and Microbial Technology, 46 (3-4), 237-245 (2010).
Tools for Enzyme Discovery -“Industrial enzymes, biocatalysis and enzyme evolution”- In "Manual of Industrial Microbiology and Biotechnology" (3rd Edition), Ed.: Richard H. Baltz, Julian E. Davies, and Arnold Demain (American Society for Microbiology) pp 441-452 (2010).
Phenylalanine Dehydrogenase. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), revised version (2010), in press.
Y. Asano and H. Komeda
D-Aminopeptidase and Alkaline D-peptidase. Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation (John Wiley & Sons), revised version (2010), in press.
"Acrylamide", "Inosininc acid", "Guanylic acid" (in Japanese), Dictionary of Biology, (Asakura Press) P10, P96, P334 (2010).
Enzymatic synthesis of D-amino acids (in Japanese), "Handbook of enzyme utilization"(NTS Inc.) pp 623-631 (2010).
A new (R)-hydroxynitrile lyase from Prunus mume for asymmetric synthesis of cyanohydrins, John Whittall and Peter Sutton (eds.). In Practical Methods in Biocatalysis and Biotransformations, Catalysts for the Fine Chemical Industry, pp 269-272 (2010).
Yasuhisa Asano, Hiroyuki Akita, and Kaoru Nakamura
Selected abstracts from the 12th Japanese Symposium on the Chemistry of Biocatalysis, J. Molec. Catal. B: Enzymatic., 62 (1), 108-126 (2010).
Hitomi Sawai, Hiroshi Sugimoto, Yasuo Kato, Yasuhisa Asano, Yoshitsugu Shiro, and Shigetoshi Aono
X-ray crystal structure of Michaelis complex of aldoxime dehydratase
J. Biol. Chem., 284 (46), 32089-32096 (2009).
I. Kira, Y. Asano and K. Yokozeki
Screening, purification, and identification of the enzyme producing N-(L-a-L-aspartyl)-L-phenylalanine methyl ester from L-isoasparagine and L-phenylalanine methyl ester, J. Biosci. Bioeng., 108 (3), 190-193 (2009).
Yasuhisa Fukuta, Hidenobu Komeda, Yoichi Yoshida, and Yasuhisa Asano
High yield synthesis of 12-aminolauric acid by "Enzymatic transcrystallization" of w-laurolactam using w-laurolactam hydrolase from Acidovorax sp.T31, Biosci. Biotech. Biochem., 73 (5), 980-986 (2009).
Shinjiro Tachibana, Yuko Kuwamori, and Yasuhisa Asano
Discrimination of aliphatic substrates by a single amino acid substitution in Bacillus badius and Bacillus sphaericus phenylalanine dehydrogenases, Biosci. Biotech. Biochem., 73 (3), 729-732 (2009).
Seiji Okazaki, Atsuo Suzuki, Tsunehiro Mizushima, Takeshi Kawano, Hidenobu Komeda, Yasuhisa Asano, and Takashi Yamane
The novel structure of a pyridoxal 5'-phosphate-dependent fold-type I racemase, a-amino-e-caprolactam racemase from Achromobacter obae, Biochemistry, 48 (5), 941-950 (2009).
T. Ueatrongchit, H. Komeda, Y. Asano, and A. H-Kittikun
Parameters influencing asymmetric synthesis of (R)-mandelonitrile by a novel (R)-hydroxynitrile lyase from Eriobotrya japonica, J. Molec. Catal. B: Enzymatic, 56 (4), 208-214 (2009).
Seiji Okazaki, Takashi Yamane, and Yasuhisa Asano
Dynamic kinetic resolution of amino acid amides to form chiral amino acids ―structure and function of a-amino-e-caprolactam racemase― (in Japanese), Protein, Nucleic Acids, and Enzyme (Tanpakushitsu, Kakusan, Koso), 54 (11), 1389-1394 (2009).
Screening for new functions of enzymes and their industrial development, Bioscience and Industry (in Japanese), 67 (1), 12-18 (2009).
N. Wehofsky, A. Pech, S. Liebscher, S. Schmidt, H. Komeda, Y. Asano, and F. Bordusa
D-amino acid specific proteases and native all-L-proteins: a convenient combination for semisynthesis, Angew. Chem. Int. Ed. Engl., 47 (29) 5456-5460 (2008).
Yasuhisa Asano, Yasuhisa Fukuta, Yoichi Yoshida, and Hidenobu Komeda
The screening, characterization, and use of w-laurolactam hydrolase: A new enzymatic synthesis of 12-aminolauric acid, Biosci. Biotech. Biochem., 72 (8) 2141-2150 (2008).
Hidenobu Komeda and Yasuhisa Asano
A novel D-stereoselective amino acid amidase from Brevibacterium iodinum: Gene cloning, expression and characterization, Enzyme and Microbial Technology, 43 (3), 276-283 (2008).
T. Ueatrongchit, A. Kayo, H. Komeda, Y. Asano, and A. H-Kittikun
Purification and characterization of a novel (R)-hydroxynitrile lyase from Eriobotrya japonica (loquat), Biosci. Biotech. Biochem., 72 (6), 1513-1522 (2008).
Seiji Okazaki, Atsuo Suzuki, Hidenobu Komeda, Yasuhisa Asano, Takashi Yamane
Deduced catalytic mechanism of D-amino acid amidase from Ochrobactrum anthropi SV3, J. Synchrotron Rad, 15, 250-253 (2008).
Seiji Okazaki, Atsuo Suzuki, Tsunehiro Mizushima, Hidenobu Komeda, Yasuhisa Asano, Takashi Yamane
Structures of D-amino acid amidase complexed with L-phenylalanine and with L-phenylalanine amide: Insight into the D-stereospecificity of D-amino acid amidase from Ochrobactrum anthropi SV3, Acta Cryst., D64, 331-334 (2008).
Reynaldo Villalonga, Akira Fujii, Hiroaki Shinohara, Shinjiro Tachibana, and Yasuhisa Asano
Covalent immobilization of phenylalanine dehydrogenase on cellulose membrane for biosensor construction. Sens. Actuators B: Chem., 129, 195-199 (2008).
Koichi Wada, and Yasuhisa Asano
Studies on the microbial production of pyridoxine 5'-a-glucoside (in Japanese), Fine Chemicals, (in Japanese), 37 (1), 36-43 (2008).
Enzymatin production of D-amino acids (in Japanese), "Microbial Bioconversion and Bioproduction -Development of White Biotechnology Beyond Chemical Synthesis"(2008), (Supervisor: Mitsuyoshi Ueda, CMC Press), pp 68-77.
Yasuhisa Asano, Hidenobu Komeda, Seiji Okazaki, Takashi Yamane
Discovery of amino acid amidases, and their use in kinetic resolution of amino acid amides, Seikagaku (in Japanese), 80 (4), 294-299 (2008).
"Dr. Jyokichi Takamine, an Agricultural Chemist", Foreword to Chemistry and Biology, Chemistry and Biology (in Japanese), 46 (4), 223 (2008).
Enzyme handbook, (EC 126.96.36.199, 188.8.131.52, 184.108.40.206, 220.127.116.11, 18.104.22.168, 22.214.171.124, 126.96.36.199, 188.8.131.52, 184.108.40.206) (in Japanese)(Asakura Press)
Yasuhisa Asano and Yasuo Kato
Enzyme handbook, (EC 220.127.116.11) (in Japanese)(Asakura Press)
Yasuhisa Asano and Hidenobu Komeda
Enzyme handbook, (EC 18.104.22.168, 22.214.171.124, 126.96.36.199, 188.8.131.52, 184.108.40.206) (in Japanese)(Asakura Press)
S. Yamaguchi, H. Komeda, and Y. Asano
New enzymatic method of chiral amino acid synthesis by dynamic kinetic resolution of amino acid amides: use of stereoselective amino acid amidases in the presence of α-amino-ε-caprolactam racemase, Appl. Environ. Microbiol., 73 (16), 5370-5373 (2007).
Yasuo Kato, Takashi Tsuda, and Yasuhisa Asano
Purification and partial characterization of N-hydroxy-L-phenylalanine decarboxylase/oxidase from Bacillus sp. strain OxB-1, an enzyme involved in aldoxime biosynthesis in the "aldoxime-nitrile pathway". Biochim. Biophys. Acta, 1774 (7), 856-865 (2007).
Seiji Okazaki, Atsuo Suzuki, Hidenobu Komeda, Shigenori Yamaguchi, Yasuhisa Asano, and Takashi Yamane
Crystal structure and functional characterization of a D-stereospecific amino acid amidase from Ochrobactrum anthropi SV3, a new member of the penicillin-recognizing proteins, J. Mol. Biol. 368 (1), 79-91 (2007).
R. Villalonga, A. Fujii, H. Shinohara, Y. Asano, R. Cao, S. Tachibana, and P. Ortiz
Supramolecular-mediated immobilization of L-phenylalanine dehydrogenase on cyclodextrin-coated Au electrodes for biosensor applications, Biotechnol. Lett. 29 (3), 447-452 (2007).
Reynaldo Villalonga, Shinjiro Tachibana, Roberto Cao, Madyu Matos, and Yasuhisa Asano
Glycosidation of phenylalanine dehydrogenase with O-carboxymethyl-poly-b-cyclodextrin, Enzyme Microb. Technol., 40 (3), 471-475 (2007).
Ei-ichiro Suzuki, Kohki Ishikawa, Yasuhiro Mihara, Nobuhisa Shimba, and Yasuhisa Asano
Structural-based engineering for transferases to improve the industrial production of 5'-nucleotide, Bull. Chem. Soc. Jpn., 80 (2), 276-286 (2007).
Yasuhisa Asano, and Sakayu Shimizu
Preface on the special issue of "Directed evolution of biocatalysts and thier uses in industry",Seibutsu-Kogaku Kaishi (in Japanese), 85 (9), 393 (2007)
Yasuhisa Asano, and Mizue Yamazaki
Development of a plant enzyme hydroxynitrile-lyase and its functional expression in Escherichia coli. Seibutsu-Kogaku Kaishi (in Japanese), 85 (9), (2007), 406-408.
Creation of methionine dehydrogenase for homocystinuria diagnosis
The collections of Technology-Transfer examples twoards Innovation to improve the quality of life with the "knowledge and wisdom" of universities, in dependent administrative agencies, and technical colleges
Research environment and industrial division, research promotion beaureau, Ministry of Education, Culture, Sports, Science and Technology (MEXT) (in English and Japanese), pp 8-9 (June 2007).
Discovery of amino acid racemase activity and its use in the dynamic kinetic resolution of amino acid amides, Fine Chemicals, (in Japanese), 36 (5), 52-59 (2007).
Enzymes acting on D-amino acid amides, R. Konno, H. Brueckner, A. D'Aniello, G. Fisher, N. Fujii, and H. Homma (eds.). In D-Amino acids: A new frontier in amino acid and protein research -practical methods and protocols-, Hauppauge, New York, U.S.A., pp 579-589 (2007).
Enzymes in aldoxime-nitrile pathway: versatile tools in biocatalysis, Tomoko Matsuda (ed.), In Future directions in bioctalysis, Elsevier, New York, U.S.A., pp 129-139 (2007).
Yasuhisa Asano, Shokichi Ohuchi, and Kohji Ishihara
Selected abstract from the 10th Japanese Symposium on the Chemistry of Biocatalysis, J. Molec. Catal. B: Enzymatic., 48 (3-4), 99-117 (2007).
Katsuaki Kobayashi, Minoru Kubo, Shiro Yoshioka, Teizo Kitagawa, Yasuo Kato, Yasuhisa Asano, and Shigetoshi Aono
Systematic regulation of the enzymatic activity of phenylacetaldoxime dehydratase by exogenous ligands, ChemBioChem., 7 (12), 2004-2009 (2006).
S. Tachibana, M. Suzuki, and Y. Asano
Application of an enzyme chip to the microquantification of L-phenylalanine, Anal. Biochem., 359 (1), 72-78 (2006).
R. Villalonga, S.Tachibana, R. Cao, P.D. Ortiz, L. Gomez, and Y. Asano
Supramolecular-mediated immobilisation of L-phenylalanine dehydrogenase on b-cyclodextrin-modified gold nanospheres, J. Experimental Nanoscience, 1 (2), 249-260 (2006).
K. Kobayashi, B. Pal, S. Yoshioka, Y. Kato, Y. Asano, T. Kitagawa, and S. Aono
Spectroscopic and substrate binding properties of heme-containing aldoxime dehydratases, OxdB and OxdRE, J. Inorganic Biochem., 100 (5-6), 1069-1074 (2006).
Reynaldo Villalonga, Shinjiro Tachibana, Roberto Cao, Hector L. Ramirez, and Yasuhisa Asano
Supramolecular-mediated thermostabilization of phenylalanine dehydrogenase modified with β-cyclodextrin derivatives, Biochem. Eng. J., 30 (1), 26-32 (2006).
Samik Nanda, Yasuo Kato, and Yasuhisa Asano
PmHNL catalyzed synthesis of (R)-cyanohydrins derived from aliphatic aldehydes, Tetrahedron: Asymmetry, 17 (5), 735-741 (2006).
H. Komeda, N. Hariyama and Y. Asano
L-Stereoselective amino acid amidase with broad substrate specificity from Brevundimonas diminuta: characterization of a new member of the leucine aminopeptidase family, Appl. Microbiol. Biotechnol., 70 (4), 412-421 (2006).
Y. Kato and Y. Asano
Molecular and enzymatic analysis of the "aldoxime-nitrile pathway" in the glutaronitrile degrader Pseudomonas sp. K-9, Appl. Microbiol. Biotechnol., 70 (1), 92-101 (2006).
Yasuhisa Asano, Hideo Hirohara, and Kohji Ishihara
Selected abstracts from the 9th Japanese Symposium on the Chemistry of Biocatalysis, J. Molec. Catal. B: Enzymatic., 42 (3-4), 114-135 (2006).
J. Sharma, D. Batovska, Y. Kuwamori, and Y. Asano
Enzymatic chemo selective synthesis of secondary-amide surfactant from N-methylethanol amine, J. Biosci. Bioeng., 100 (6), 662-666 (2005). [pdf]
Y. Asano, K. Tamura, N. Doi, T. Ueatrongchit, A. H-Kittikun, and T. Ohmiya
Screening for new hydroxynitrilases from plants, Biosci. Biotech. Biochem., 69 (12), 2349-2357 (2005).
Y. Kato, and Y. Asano
Purification and characterization of aldoxime dehydratase of the head blight fungus, Fusarium graminearum, Biosci. Biotech. Biochem., 69 (11), 2254-2257 (2005).
S. Nanda, Y. Kato, and Y. Asano
A new (R)-hydroxynitrile lyase from Prunus mume: asymmetric synthesis of cyanohydrins, Tetrahedron, 61 (46), 10908-10916 (2005).
Y. Asano and S. Yamaguchi
Discovery of amino acid amides as new substrates for a-amino-e-caprolactam racemase from Achromobacter obae, J. Molec. Catal. B: Enzymatic., 36 (1-6), 22-29 (2005).
R. Villalonga, S. Tachibana, Y. Perez, and Y. Asano
Increased conformational and thermal stability properties for phenylalanine dehydrogenase by chemical glycosidation with end-group activated dextran, Biotechnol. Lett., 27 (17), 1311-1317 (2005).
H. Komeda and Y. Asano
A DmpA-homologous protein from Pseudomonas sp. is a dipeptidase specific for β-alanyl dipeptides.